Increased production of ginsenosides through improvement of protein-folding machinery of yeast
AssigneeKorea Research Institute of Chemical Technology
InventorsSun-Chang Kim, Ju Young Lee, In Seung Jang, Seohyun Kim, Jong Geon Jegal
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Abstract
The present invention relates to recombinant yeast, in which the productivity of ginsenoside is enhanced by overexpressing CPR5, PDI1, or ERO1 in yeast having the productivity of ginsenosides; a method for preparing the yeast; and a method for producing ginsenosides using the yeast.
Claims17
A recombinant yeast for producing a ginsenoside or a precursor thereof, wherein the recombinant yeast expresses a polynucleotide that encodes a protein involved in protein folding having at least 95% sequence homology to SEQ ID NO:1 or SEQ ID NO:3,
- wherein the protein involved in protein folding has Cyclosporin-sensitive Proline Rotamase 5 (CPR5)-like activity, or ER Oxidation or Endoplasmic Reticulum Oxidoreductin (ERO1)-like activity;
- wherein the recombinant yeast has elevated expression levels of the protein involved in protein folding in comparison to endogenous expression levels of the protein;
- and
- wherein the recombinant yeast comprises ginsenoside synthesizing genes encoding the proteins of HMG-CoA reductase (tHMG1), Panax ginseng squalene epoxidase (PgSE), Panax ginseng dammarenediol-ll synthase (PgDDS), Panax ginseng cytochrome P450 CYP716A47 (PgPPDS), and Panax ginseng NADPH-cytochrome P450 reductase (PgCPR).
A method for preparing recombinant yeast with an enhanced productivity of ginsenosides compared to parent yeast, comprising transforming the recombinant yeast cell with a polynucleotide having the sequence of SEQ ID NO:1 and/or SEQ ID NO:3 such that expression of a protein encoded by the polynucleotide is increased, and wherein the protein has CPR5-like activity or ERO1-like activity in a ginsenoside-producing yeast strain relative to its endogenous expression levels.
A method for preparing recombinant yeast with an enhanced productivity of ginsenoside precursors compared to parent yeast, comprising increasing the expression level of a protein encoded by the sequence of SEQ ID NO:1 and/or 3, and wherein the protein has CPR5-like activity or ERO1-like activity in a ginsenoside precursor-producing yeast strain relative to its endogenous expression levels.
Dependencies are inferred from the claim text.