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US 11,046,986 B2Granted
Method for producing RNA
AssigneeAjinomoto Co., Inc.
InventorsShuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
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Abstract
A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
Claims10
A method for producing objective RNA, the method comprising:
- A) culturing a coryneform bacterium having an expression unit for the objective RNA in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium;
- and
- B) collecting the objective RNA from the cells,
- wherein the bacterium has been modified so that the activity of ribonuclease III is reduced as compared with a non-modified strain by deletion of a gene encoding ribonuclease III;
- wherein the ribonuclease III is a protein selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 52;
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 52, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having ribonuclease III activity;
- and
- (c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 52, and having ribonuclease III activity.
9 more elements
Dependencies are inferred from the claim text.