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US 11,046,984 B2Granted
Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides
AssigneeGlycosyn LLC
InventorsJohn M. McCoy, Matthew Ian Heidtman, Massimo Merighi
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Abstract
The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Claims31
A method for producing a fucosylated lacto-N-fucopentaose I (LNF I) oligosaccharide in a bacterium comprising
- providing a bacterium comprising an exogenous lactose-utilizing α(1,2) fucosyltransferase enzyme, wherein said α(1,2) fucosyltransferase enzyme has at least 90% sequence identity to amino acid sequence SEQ ID NO:
- 13;
- and
- culturing said bacterium in the presence of lactose, wherein said LNF I oligosaccharide is produced,
- wherein said bacterium further comprises β1,3-N-acetylglucosaminyltransferase and β1,3-galactosyltransferase.
2 more elements
Dependencies are inferred from the claim text.