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US 11,046,962 B2Granted
Lux expression in cells and methods of use
OwnerDaniel Close, Steven Ripp +2inventors
InventorsDaniel Close, Steven Ripp, Gary Sayler, Michael Conway
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OwnerDaniel Close, Steven Ripp +2inventors
InventorsDaniel Close, Steven Ripp, Gary Sayler, Michael Conway
Estimating the term…
Cells, including stem cells, comprising an autobioluminescent phenotype, wherein the cells emit a luminescent signal in the absence of an exogenous luminescent stimulator, are provided. The luminescent signal may be constitutive, inducible, repressible, or tissue-specific. The cells express a synthetically engineered bacterial luciferase (lux) cassette, i.e., the luxCDABEfrp gene cassette. The cells may comprise luxA, luxB, luxC, luxD, luxE, and flavin reductase. The cells may each express a combined expression level of luxC, luxD, luxE, and flavin reductase that is from ten to forty times greater than a combined expression level of luxA and luxB. Further, methods of making and using the cells comprising an autobioluminescent phenotype are disclosed herein.
A stem cell comprising an autobioluminescent phenotype including a luminescent signal in the absence of an exogenous luminescence stimulator, and wherein the stem cell further comprises:
A stem cell comprising an autobioluminescent phenotype including a luminescent signal in the absence of an exogenous luminescence stimulator, the stem cell further comprising:
A cell comprising an autobioluminescent phenotype including a luminescent signal in the absence of an exogenous luminescence stimulator, wherein the cell expresses luxA, luxB, luxC, luxD, luxE, and flavin reductase, and wherein at least one of luxC, luxD, luxE, or flavin reductase is expressed in a molar ratio of at least ten times greater than at least one of luxA or luxB.
A cell comprising an autobioluminescent phenotype including a luminescent signal in the absence of an exogenous luminescence stimulator, wherein the cell comprises at least two vectors having at least one of a luxA nucleic acid, a luxB nucleic acid, a luxC nucleic acid, a luxD nucleic acid, a luxE nucleic acid, or a flavin reductase nucleic acid, and wherein the luxA nucleic acid and the luxB nucleic acid are on different of the at least two vectors and are driven by one or more promotors having strong transcriptional activity.
Dependencies are inferred from the claim text.