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US 11,046,953 B2Granted
High efficiency, small volume nucleic acid synthesis
AssigneesLife Technologies Corporation, Thermo Fisher Scientific Geneart GmbH
InventorsTodd Peterson, Axel Trefzer, Thomas Poehmerer
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Abstract
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
Claims18
A method for producing a plurality of nucleic acid molecules, the method comprising:
- (a) synthesizing the plurality of nucleic acid molecules on separate beads in wells of a plate, wherein each well is configured to accommodate a single bead, wherein the volume of each well is between 0.1 picoliters and 10 nanoliters, wherein the wells comprise an electrode for the production of electrochemically generated acid, and wherein the nucleic acid molecules synthesized on each bead are designed to have the same nucleotide sequence;
- (b) selectively removing a plurality of beads from the wells, wherein the beads removed from the wells comprise a first set of individual nucleic acid molecules, wherein beads comprising nucleic acid molecules that are not part of the first set of individual nucleic acid molecules are not removed from their respective wells;
- and
- (c) collecting the beads selectively removed from wells in step (b) in a vessel.
1 more element
Dependencies are inferred from the claim text.