# US 11,041,854 B2 · Biosensor using magnetic nanoparticles, and detection device and detection method using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,041,854 B2 - Document ID: US11041854B2 - Status: Granted - Kind: B2 - Application number: 17/002,632 - Filed: 2020-08-25 - Published: 2021-02-04 - Granted: 2021-06-22 - Earliest claimed: 2018-08-09 - Assignees: Bbb Inc. - Applicants: Bbc Inc. - Inventors: Hyundoo Hwang; Jaekyu Choi - Web page: https://patentgrep.com/patent/US11041854B2 ## CPC - G01N 33/54333: {Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction} - G01N 27/3277: {being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48)} - G01N 27/3278: {involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745)} - G01N 33/54346: {Nanoparticles} - G01N 33/5438: {Electrodes} - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - G01N 33/553: Metal or metal coated - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract According to one embodiment of the present application, a biosensor, the biosensor comprising: a reaction part including a magnetic nanoparticle complex, a first electrode, and a second electrode; and a sample introduction part forming a passage so that a sample can be introduced into the reaction part from an outside of the biosensor; wherein the magnetic nanoparticle complex includes a first capturing substance for capturing a first target substance, a magnetic nanoparticle, and a reaction substance that performs at least one of an oxidation reaction and a reduction reaction, wherein the magnetic nanoparticle complex has is magnetic in the reaction part, and has a property that mobility can be changed according to a change in a condition of the reaction part, wherein the first electrode, a second capturing substance for capturing a second target substance is fixed, wherein the second electrode is an electrode different from the first electrode, and characterized in that at least one of the first target substance and the second target substance is included in the sample, may be provided. ## Claims ### Claim 1 A biosensor used to detect whether a target substance is included in a sample based on a change of a current in response to a change of a voltage applied between a first electrode and a second electrode, the biosensor comprising: a reaction part including a magnetic nanoparticle complex, the first electrode, and the second electrode; and a sample introduction part forming a passage so that the sample can be introduced into the reaction part from an outside of the biosensor; wherein the magnetic nanoparticle complex includes a magnetic nanoparticle, and a reaction substance is fixed to the magnetic nanoparticle, and a first capturing substance is fixed to the reaction substance, wherein the first capturing substance is configured to capture the target substance or is configured to capture a second capturing substance, wherein the reaction substance performs at least one reaction selected from an oxidation reaction and a reduction reaction, wherein the magnetic nanoparticle complex is magnetic in the reaction part, and has a property that a movement of the magnetic nanoparticle complex is changed according to a change in a condition of the reaction part, wherein the second capturing substance is configured to capture the target substance, and is fixed to the first electrode, wherein the second electrode is an electrode different from the first electrode, and wherein, when the first capturing substance of the magnetic nanoparticle complex is captured by the second capturing substance of the first electrode directly or indirectly, the reaction of the reaction substance induces the change of the current in response to a voltage change applied between the first electrode and the second electrode to represent existence of the target substance included in the sample. ### Claim 2 (depends on 1) The biosensor of claim 1, wherein the reaction substance is fixed to a reactor formed on a surface of the magnetic nanoparticle. ### Claim 3 (depends on 2) The biosensor of claim 2, wherein the reactor is an amine, and the reaction substance is gold. ### Claim 4 (depends on 1) The biosensor of claim 1, wherein a blocking substance that prevents adhesion of the target substance is located on of at least a portion of the first electrode. ### Claim 5 (depends on 4) The biosensor of claim 4, wherein the blocking substance is a BSA (Bovine Serum Albumin). ### Claim 6 (depends on 1) The biosensor of claim 1, wherein the first capturing substance includes at least one selected from an antigen, an antibody, a modified antibody, an antibody analog, an aptamer, a nucleic acid, a lipid, and a viral protein antigen, and wherein the second capturing substance includes at least one of an antigen, an antibody, a modified antibody, an antibody analog, an aptamer, a nucleic acid, a lipid, and a viral protein antigen. ### Claim 7 (depends on 1) The biosensor of claim 1, wherein the first capturing substance is an antibody, the target substance is an antigen interacting with the first capturing substance, the second capturing substance is same substance with the first capturing substance, wherein when the target substance is included in the sample, the second capturing substance bonds to the target substance and the target substance bonds to the first capturing substance such that the first capturing substance is captured by the second capturing substance indirectly. ### Claim 8 (depends on 1) The biosensor of claim 1, wherein the first capturing substance is an antigen, the second capturing substance is an antibody interacting with the first capturing substance, the target substance is same substance with the first capturing substance, wherein when the target substance is included in the sample, the second capturing substance bonds to the first capturing substance competitively with the target substance such that the first capturing substance is captured by the second capturing substance directly. ### Claim 9 (depends on 1) The biosensor of claim 1, wherein the first capturing substance is an antigen, the target substance is an antibody interacting with the first capturing substance, the second capturing substance is same substance with the first capturing substance, wherein when the target substance is included in the sample, the second capturing substance bonds to the target substance and the target substance bonds to the first capturing substance such that the first capturing substance is captured by the second capturing substance indirectly. ### Claim 10 (depends on 1) The biosensor of claim 1, when a magnetic field formed in the reaction part is changed, wherein a direction of the movement of the magnetic nanoparticle complex is changed. ### Claim 11 (depends on 1) The biosensor of claim 1, when the voltage applied between the first electrode and the second electrode is changed, wherein a direction of the movement of the magnetic nanoparticle complex is changed. ### Claim 12 (depends on 1) The biosensor of claim 1, further comprising a third electrode having a coil shape different from the first electrode and the second electrode, and when a current applied to the third electrode is changed, wherein a direction of the movement of the magnetic nanoparticle complex is changed. ### Claim 13 (depends on 1) The biosensor of claim 1, wherein at least a portion of a fourth electrode electrically connected to the first electrode and at least a portion of a fifth electrode electrically connected to the second electrode are exposed to the outside of the biosensor. ### Claim 14 (depends on 13) The biosensor of claim 13, wherein the first electrode and the fourth electrode are composed of one electrode, and wherein the second electrode and the fifth electrode are composed of one electrode. ### Claim 15 (depends on 13) The biosensor of claim 13, wherein at least a portion of the fourth electrode and at least a portion of the fifth electrode are electrically connected to a device capable of measuring a current. ### Claim 16 (depends on 15) The biosensor of claim 15, wherein the voltage applied between the first electrode and the second electrode is controlled by the device. ### Claim 17 (depends on 1) The biosensor of claim 1, wherein the first capturing substance is an antibody, the second capturing substance is an antigen interacting with the first capturing substance, the target substance is same substance with the first capturing substance, wherein when the target substance is included in the sample, the second capturing substance bonds to the first capturing substance competitively with the target substance such that the first capturing substance is captured by the second capturing substance directly.