Genetic markers for discrimination and detection of red sea bream iridovirus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same

A kit for discrimination or detection of Red sea bream iridovirus (RSIV), the kit comprising:

• a primer consisting of SEQ ID NO:
• 8;
• and a PNA probe consisting of SEQ ID NO:
• 3.

A method for detecting Red sea bream iridovirus (RSIV), the method comprising the steps of:

• (a) extracting a target nucleic acid from a fish sample;
• (b) amplifying a genetic marker nucleotide sequence for Red sea bream iridovirus (RSIV) contained in the target nucleic acid by use of a primer pair capable of amplifying a fragment consisting of SEQ ID NO:
• 19;
• (c) producing a single-strand genetic marker sequence fragment using the amplified genetic marker nucleotide sequence as a template and a primer consisting of SEQ ID NO:
• 8;
• (d) hybridizing a PNA probe consisting of a reporter and quencher labeled SEQ ID NO:
• 3 to the produced single-strand genetic marker sequence fragment;
• (e) obtaining a temperature-dependent melting curve while increasing the temperature of a PNA probe-hybridized product resulting from step (d);
• and
• (f) detecting whether or not the fish sample is infected with red sea bream iridovirus (RSIV) by analyzing the melting curve obtained in step (e) to determine a melting temperature.