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US 11,041,149 B2Granted
PH20 polypeptide variants, formulations and uses thereof
AssigneeHalozyme, Inc.
InventorsGe Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
Life of this patent
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Abstract
Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.
Claims17
A modified PH20 polypeptide, comprising one or more amino acid replacements in an unmodified PH20 polypeptide, wherein:
- the unmodified PH20 polypeptide consists of the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 3, 7 and 32-66;
- the modified PH20 polypeptide exhibits increased hyaluronidase activity compared to the unmodified PH20 polypeptide not containing the amino acid replacement(s);
- the amino acid replacement(s) confer(s) the increased hyaluronidase activity;
- the amino acid replacement(s) is at a position corresponding to 313 with reference to amino acid positions of SEQ ID NO:3, which confers increased hyaluronidase activity;
- corresponding amino acid positions are identified by alignment of the PH20 polypeptide with the polypeptide having the sequence of amino acids set forth in SEQ ID NO:3;
- and
- the modified PH20 polypeptide has at least 91% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 3, 7 and 32-66.
6 more elements
A modified PH20 polypeptide, comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 591-598, or a sequence of amino acids that exhibits at least 91% sequence identity to a sequence of amino acids set forth in any of SEQ ID NOs:
- 591-598 and that contains the amino acid replacement at residue 313 compared to corresponding positions in the amino acid sequence selected from the group consisting of SEQ ID NOs:
- 3 and 32-66.
Dependencies are inferred from the claim text.