Method for preparing nicotinamide mononucleotide (NMN)

A method for preparing nicotinamide mononucleotide (NMN), comprising:

• catalytically reacting a plurality of raw materials comprising nicotinamide, ATP, and ribose in the presence of nicotinamide phosphoribosyltransferase (Nampt), ribose phosphate pyrophosphokinase, and ribokinase, to prepare the NMN,
• wherein the Nampt is a protein derived from a protein having the amino acid sequence of SEQ ID NO:
• 2, wherein the amino acid sequence of the Nampt differs from the amino acid sequence of SEQ ID NO:
• 2 in that the Nampt has a mutation;
• wherein the mutation is selected from the group consisting of F180A, F180W, A182Y, E231A, E231Q, D298A, D298N, D298E, D338N, D338E, D377A, D377N, D377E, a combination of E231Q and D338E, a combination of E231Q and D377E, a combination of D338E and D377E, a combination of E231Q, D338E and D377E, and a combination of E231Q, D298A, D338E and D377E.