Methods in digital counting
AssigneeSelma Diagnostics Aps
InventorAndreas Hjarne Kunding
Life of this patent
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Abstract
The present invention relates to methods and systems for testing for the presence of a material such as one or more analyte types within a sample and more particularly, for improved single enzyme-linked immunosorbent assay (sELISA) testing as well as other variants of single-enzyme linked molecular analysis (SELMA). Background and false positives are reduced due to the presence of at least two detection cycles where each detection cycle comprises the steps of a) triggering a signal from captured and labelled analyte(s), b) recording of the number and positions of capture sites exhibiting a signal from the captured and labelled analyte(s), c) and before a further detection cycle is performed, deactivation of signal(s).
Claims16
A method for digital counting analysis of a sample potentially containing at least one analyte, wherein the sample has been contacted with a solid phase having a plurality of discrete capture sites, each site being capable of capturing the at least one analyte, which method comprises at least two detection cycles in which the same analyte is labelled or re-labelled with a labelling agent, where each detection cycle comprises the steps of
- a) triggering a signal from captured and labelled or re-labelled analyte(s),
- b) recordation of the number and positions of capture sites exhibiting a signal from the captured and labelled or re-labelled analyte(s), and
- c) before a further detection cycle is performed, deactivation of the signal(s)
- wherein the step of signal deactivation is selected from
- i. detaching and removing the labelling agent from the captured analyte,
- ii. deactivating the ability of the labelling agent to facilitate a signal or
- iii. a combination of i) and ii).
A method for digital counting analysis of a sample containing more than one analyte types, wherein the sample has been contacted with a solid phase having a plurality of discrete capture sites, each site being capable of capturing a specific analyte type, which method comprises at least two detection cycles in which at least one of the analyte types is labelled or relabeled with a labelling agent, where each detection cycle comprises the steps of
- a) triggering a signal from captured and labelled or re-labelled analyte(s),
- b) recordation of the number and positions of capture sites exhibiting a signal from the captured and labelled or re-labelled analyte(s), and
- c) before a further detection cycle is performed, deactivating the signal(s)
- wherein the step of signal deactivation is selected from
- i. detaching and removing the labelling agent from the captured analyte,
- ii. deactivating the ability of the labelling agent to facilitate a signal or
- iii. a combination of i) and ii).
Dependencies are inferred from the claim text.