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US 11,034,990 B2Granted
Optimized host cells for the production of glutathione
AssigneeLallemand SAS
InventorsCaroline Wilde, Corinne Cluis
Life of this patent
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Abstract
The disclosure concerns a genetically modified host cell for the production and accumulation of glutathione (GSH). The genetically modified host cell can allow the expression of a mutated Cys4p whose activity is increased. In addition or alternatively, the genetically modified host cell can express a mutated Yap1p whose translocation from the nucleus to the cytoplasm is reduced. Furthermore, in addition or alternatively, the genetically modified host cell can express an heterologous threonine aldolase (Gly1p).
Claims37
A genetically modified host cell comprising at least two of:
- a first heterologous nucleic acid molecule encoding a mutated cystathionine beta-synthase protein (Cys4p) having an increased biological activity when compared to a wild-type Cys4p, wherein the mutated Cys4p is a fragment of the wild-type Cys4p;
- a second heterologous nucleic acid molecule encoding a mutated Yap1p having a reduced ability of being translocated from nucleus to cytoplasm of the genetically modified host cell when compared to a wild-type Yap1p, wherein the mutated Yap1p has at least one amino acid substitution when compared to the wild-type Yap1p;
- and
- a third heterologous nucleic acid molecule coding for a threonine aldolase protein (Gly1p), a variant of the Gly1p having threonine aldolase activity or a fragment of the Gly1p having threonine aldolase activity.
1 more element
Dependencies are inferred from the claim text.