Microbial engineering for the production of isoprenoids
AssigneeMassachusetts Institute of Technology
InventorsSteven McBride Edgar, Alkiviadis Orfefs Chatzivasileiou, Valerie Ward, Gregory Stephanopoulos
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Abstract
Disclosed herein are engineered cells and cell-free systems, compositions, and methods for conversion of isopentenols to isoprenoid precursors.
Claims14
A host cell engineered to recombinantly express polynucleotides encoding Isopentenol Utilization Pathway (IUP) enzymes producing isoprenoid precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) from isoprenol and/or prenol, wherein the IUP enzymes comprise one or more heterologous enzymes, and the IUP enzymes comprise a Saccharomyces cerevisiae choline kinase, an isopentenyl phosphate kinase, and an isopentenyl pyrophosphate isomerase (IDI), and wherein when the host cell is Saccharomyces cerevisiae, the Saccharomyces cerevisiae host cell is transformed with an exogenous polynucleotide encoding the Saccharomyces cerevisiae choline kinase.
Dependencies are inferred from the claim text.