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US 11,034,931 B2Granted
Yeast for preparing alcoholic beverages
AssigneeCarlsberg Breweries A/S
InventorsNatalia Y. Solodovnikova, Jeppe Frank Andersen, Rosa Garcia Sanchez, Zoran Gojkovic
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Abstract
The invention relates to yeast cells with useful characteristics, including being capable of utilizing panose as sole carbon source and/or capable of utilizing one or more dipeptidesas sole nitrogen source. The invention also relates to yeast cells with useful genotypes including comprising at least 4 allelic genes encoding IMA1p and/or at least two allelic genes encoding IMA5p.
Claims17
A yeast cell having each of the following characteristics:
- characteristic II:
- capable of utilizing panose as sole carbon source;
- and
- characteristic VIII:
- capable of utilizing melibiose as sole carbon source;
- wherein the yeast cell comprises nucleotide sequences of at least 3 genes each individually encoding an IMA1p, wherein the IMA1p is selected from the group consisting of:
- an IMA1p comprising the amino acid sequence of SEQ ID NO:
- 12, an IMA1p comprising the amino acid sequence of SEQ ID NO:
- 13, an IMA1p of SEQ ID NO:
- 14, an IMA1p comprising the amino acid sequence of SEQ ID NO:
- 15, an IMA1p comprising the amino acid sequence of SEQ ID NO:21, an IMA1p comprising the amino acid sequence of SEQ ID NO:22, an IMA1p comprising the amino acid sequence of SEQ ID NO:23, an IMA1p comprising the amino acid sequence of SEQ ID NO:24, an IMA1p comprising the amino acid sequence of SEQ ID NO:25, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 12, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 13, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 14, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 15, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 21, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 22, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 23, an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 24, and an IMA1p comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 25;
- and wherein the yeast cell comprises nucleotide sequences of at least 2 genes each individually encoding an AGT1, wherein the AGT1 is selected from the group consisting of:
- an AGT1 comprising the amino acid sequence of SEQ ID NO:
- 18, an AGT1 comprising the amino acid sequence of SEQ ID NO:
- 19, an AGT1 comprising the amino acid sequence of SEQ ID NO:20, an AGT1 comprising the amino acid sequence of SEQ ID NO:27, an AGT1 comprising the amino acid sequence of SEQ ID NO:28, an AGT1 comprising the amino acid sequence of SEQ ID NO:30, an AGT1 comprising the amino acid sequence of SEQ ID NO:31, an AGT1 comprising the amino acid sequence of SEQ ID NO:32, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 18, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 19, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 20, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 27, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 28, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 30, an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 31, and an AGT1 comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 32.
29 more elements
Dependencies are inferred from the claim text.