Duck hepatitis A virus type 3 mutant CH-P60-117C and construction thereof
AssigneeSichuan Agricultural University
InventorsAnchun Cheng, Xingjian Wen, Mingshu Wang, Liping Wu
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Abstract
Disclosed herein are a duck hepatitis A virus type 3 (DHAV-3) mutant CH-P60-117C and a construction method thereof. The DHAV-3 mutant CH-P60-117C is constructed by mutating A at position 117 of 5′-UTR of the genome of the DHAV-3 virulent strain to C; mutating T at position 1142 to A to mutate tyrosine-164 of VP0 protein of the parent strain to asparagine; and mutating C at position 4334 to A so that leucine-71 of the viral protein 2C of the parent strain is mutated to isoleucine.
Claims6
A duck hepatitis A virus-3 mutant CH-P60-117C (DHAV-3 CH-P60-117C), wherein the DHAV-3 mutant CH-P60-117C is deposited in the China Center for Type Culture Collection (CCTCC) on Dec. 2, 2018, with an accession number of CCTCC NO:
- V201860;
- wherein the DHAV-3 mutant CH-P60-117C is obtained by mutating A at position 117 of 5′-UTR of genome of a DHAV-3 virulent strain to C, mutating T at position 1142 to A to mutate tyrosine-164 of a VP0 protein of the DHAV-3 virulent strain to asparagine, and mutating C at position 4334 to A so that leucine-71 of protein 2C of the DHAV-3 virulent strain is mutated to isoleucine;
- wherein the DHAV-3 mutant CH-P60-117C comprises T, mutated from G, at position 3403 of the genome as a genetic marker of infectious clones;
- and wherein the DHAV-3 virulent strain is deposited in the China Center for Type Culture Collection with an accession number of CCTCC NO:
- V201305.
Dependencies are inferred from the claim text.