Zika virus vaccine and methods of production
AssigneesThe Government of the United States, As Represented by the Secretary of the Army Fort Detrick, Maryland, The Research Foundation For The State University of New York Syracuse, New York
InventorsStephen J. Thomas, Timothy Endy, Kenneth H. Eckels, J. Robert Putnak, Richard Jarman, Rafael De La Barrera
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Abstract
The invention generally relates to a purified inactivated Zika virus (ZIKV), methods for producing the purified inactivated ZIKV, immunogenic compositions and vaccines comprising the purified inactivated ZIKV and methods for the prevention and/or treatment of infection by ZIKV.
Claims17
A purified, inactivated, immunogenic Zika virus (ZIKV), wherein the purified, inactivated, immunogenic ZIKV is produced using a Zika virus strain, wherein the Zika virus strain is selected from the group consisting of:
- a purified, inactivated Puerto Rico PRVABC59 strain, a purified, inactivated Thailand SVO127/14 strain, a purified, inactivated Philippine COC C 0740 strain, and a purified, inactivated Brazil Fortaleza/2015 strain.
A method of generating a purified inactivated ZIKV comprising the steps of:
- i) inoculating a cell culture with an amount of a ZIKV strain;
- ii) growing the inoculated virus in cell culture;
- iii) harvesting and isolating virus fluids from the inoculated cell culture to prepare a Zika virus concentrate;
- iv) purifying the ZIKV concentrate;
- v) inactivating the purified ZIKV;
- and
- vi) recovering the purified, inactivated ZIKV.
Dependencies are inferred from the claim text.