Stabilization of glutamate dehydrogenase in an aqueous solution
AssigneeBiomerieux
InventorsFlorence Bettsworth, Jérôme Martinez
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Abstract
An aqueous composition includes (i) glutamate dehydrogenase from a bacterium of the Clostridium genus, (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof, and (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase. A process for stabilizing the glutamate dehydrogenase in order to maintain antigenic properties of the glutamate dehydrogenase includes stabilizing the glutamate dehydrogenase in the aqueous composition and maintaining the antigenic properties of the glutamate dehydrogenase during storage of the aqueous composition.
Claims29
A process for stabilizing glutamate dehydrogenase from a bacterium of the Clostridium genus in order to maintain antigenic properties of the glutamate dehydrogenase, comprising:
- stabilizing the glutamate dehydrogenase in an aqueous solution comprising (i) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof, and (ii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase;
- and
- maintaining the antigenic properties of the glutamate dehydrogenase during storage of the aqueous solution, wherein:
- the glutamate dehydrogenase is in the aqueous solution at a concentration ranging from 0.75 to 10 ng/ml;
- and
- the aqueous composition has a pH of between 4.5 and 7.
An aqueous composition comprising:
- (i) glutamate dehydrogenase from a bacterium of the Clostridium genus;
- (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof;
- and
- (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase, wherein:
- the glutamate dehydrogenase is in the aqueous composition at a concentration ranging from 0.75 to 10 ng/ml;
- and
- the aqueous composition has a pH of between 4.5 and 7.
Dependencies are inferred from the claim text.