# US 11,029,310 B2 · Device and method for extracting a targeted fraction from a sample

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- Number: US 11,029,310 B2
- Document ID: US11029310B2
- Status: Granted
- Kind: B2
- Application number: 13/804,122
- Filed: 2013-03-14
- Published: 2014-09-18
- Granted: 2021-06-08
- Earliest claimed: 2013-03-14
- Assignees: Wisconsin Alumni Research Foundation
- Applicants: Wisconsin Alumni Research Foundation
- Inventors: David J. Beebe; Scott M. Berry
- Web page: https://patentgrep.com/patent/US11029310B2

## CPC

- G01N 33/54326: {Magnetic particles}
- B01L 3/5085: {for multiple samples, e.g. microtitration plates}
- B01L 3/5088: {confining liquids at a location by surface tension, e.g. virtual wells on plates, wires (B01L3/50857 takes precedence)}
- B01L 3/563: {Joints or fittings; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors}
- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- G01N 1/405: {by adsorption or absorption}
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/0819: Microarrays; Biochips
- B01L 2300/161: Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L 2400/043: magnetic forces
- Y10T 436/25375: Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A device and a method for isolating a target from a sample are provided. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes a first plate having a first region for receiving at least a portion of the sample. A second plate is spaced from the first plate by a distance and has a first region for receiving a reagent. A force attracts the target bound solid phase substrate toward the first region of the second plate such that the target bound solid phase substrate in the portion of the sample are drawn through the air gap and into the reagent by the force.

## Claims

### Claim 1

A device for isolating a target from a sample, the sample including the target bound to solid phase substrate to form target bound solid phase substrate, comprising:
a first plate lying in a first plane and having a first surface, the first surface including a first region for receiving at least a portion of the sample;
a second plate lying in a second plane which is generally parallel to and spaced from the first plate along an axis generally perpendicular to the first and second planes by a distance, the second plate having a first surface directed toward the first surface of the first plate and including a first region for receiving a reagent; and
a magnetic force generator positionable adjacent the second plate, the magnetic force generator generating a magnetic force which draws the target bound solid phase substrate away from the first surface of the first plate toward the first surface of the second plate in a direction generally parallel to the axis when the portion of the sample is received on the first region of the first plate;
wherein:
when the portion of the sample is received on the first region of the first plate and when the reagent is received on the first region of the second plate, the distance between the first plate and the second plate being sufficient:
to provide an air gap between the portion of the sample received by first region of the first plate and the reagent received by the first region of the second plate; and
for the target bound solid phase substrate in the portion of the sample to be drawn through the air gap and into the reagent by the magnetic force generated by the magnetic force generator.

### Claim 2 (depends on 1)

The device of claim 1 wherein the first region of the first plate is defined by a portion of the first surface of the first plate.

### Claim 3 (depends on 2)

The device of claim 2 wherein the first region of the first plate is hydrophilic.

### Claim 4 (depends on 2)

The device of claim 2 wherein the first surface of the first plate external of the first region thereof is hydrophobic.

### Claim 5 (depends on 1)

The device of claim 1 wherein the first region of the second plate is defined by a portion of the first surface of the second plate.

### Claim 6 (depends on 5)

The device of claim 5 wherein the first region of the second plate is hydrophilic.

### Claim 7 (depends on 5)

The device of claim 5 wherein the first surface of the second plate external of the first region thereof is hydrophobic.

### Claim 8 (depends on 1)

The device of claim 1 wherein the first plate includes a second surface and wherein the first region of the first plate includes a recess in the second surface and a passageway extending between the recess and the first surface of the first plate.

### Claim 9 (depends on 1)

The device of claim 1 wherein the first region of the second plate includes a recess in the first surface of the second plate.

### Claim 10 (depends on 1)

The device of claim 1 wherein the second plate includes a second surface and wherein the magnetic force generator is a magnet positioned adjacent the second surface of the second plate for generating the magnetic force.

### Claim 11

A device for isolating a target from a sample, the sample including the target bound to solid phase substrate to form target bound solid phase substrate, comprising:
a first sample receiving region disposed on a first plate for receiving at least a portion of the sample;
a first reagent region disposed on a second plate for receiving a reagent, the first reagent region opposing the first sample receiving region; and
a magnetic force generator adjacent the second plate, the magnetic force generator generating a magnetic force for drawing the target bound solid phase substrate from the at least the portion of the sample, through an air gap and into the reagent when the at least the portion of the sample is received on the first sample receiving region of the first plate and when the reagent is received on the first reagent region of the second plate;
wherein:
the first and second plates are generally parallel to and spaced from each other along an axis generally perpendicular to the first and second planes by a distance such that when the at least the portion of the sample is received on the first sample receiving region of the first plate and when the reagent is received on the first reagent region of the second plate:
the distance between the first plate and the second plate is sufficient to provide the air gap between the at least the portion of the sample received by first sample receiving region of the first plate and the reagent received by the first reagent region of the second plate; and
the target bound solid phase substrate in the at least the portion of the sample is drawn from the at least the portion of the sample, through the air gap, and into the reagent by the magnetic force generated by the magnetic force generator.

### Claim 12 (depends on 11)

The device of claim 11 wherein the first plate includes a first surface, the first sample receiving region being defined by a portion of the first surface of the first plate.

### Claim 13 (depends on 12)

The device of claim 12 wherein the first sample receiving region of the first plate is hydrophilic and wherein the first surface of the first plate external of the first sample receiving region thereof is hydrophobic.

### Claim 14 (depends on 12)

The device of claim 12 wherein the first plate includes a second surface and wherein the first sample receiving region of the first plate includes a recess in the second surface and a passageway extending between the recess and the first surface of the first plate.

### Claim 15 (depends on 12)

The device of claim 12 wherein the first reagent region of the second plate includes a recess in the first surface of the second plate.

### Claim 16 (depends on 11)

The device of claim 11 wherein the second plate includes a first surface, the first reagent region being defined by a portion of the first surface of the second plate.

### Claim 17 (depends on 16)

The device of claim 16 wherein the first reagent region of the first plate is hydrophilic and wherein the first surface of the second plate external of the first reagent region thereof is hydrophobic.