# US 11,029,309 B2 · Composition comprising up-converting phosphors for detecting an analyte > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,029,309 B2 - Document ID: US11029309B2 - Status: Granted - Kind: B2 - Application number: 16/217,550 - Filed: 2018-12-12 - Published: 2019-04-18 - Granted: 2021-06-08 - Earliest claimed: 2013-11-27 - Assignees: Roche Diabetes Care, Inc. - Applicants: Roche Diabetes Care, Inc. - Inventors: Carina Horn; Timo-Jaakko Valta - Web page: https://patentgrep.com/patent/US11029309B2 ## CPC - G01N 33/525: {Multi-layer analytical elements} - G01N 21/77: by observing the effect on a chemical indicator - G01N 33/521: {Single-layer analytical elements} - C12Q 1/26: involving oxidoreductase - C12Q 1/32: involving dehydrogenase - C12Q 1/54: involving glucose or galactose - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract The present invention relates to a detector matrix for detecting at least one analyte in a sample, preferably a sample of a body fluid, comprising at least one enzyme active in the presence of said at least one analyte and at least one indicator reagent changing at least one optical property dependent on the activity of said enzyme, wherein said detector matrix further comprises up-converting phosphor particles, preferably UV-emitting up-converting phosphor particles. The invention further relates to a test element and a test device for detecting at least one analyte in a sample comprising the detector matrix of the invention, as well as to a method for the manufacture of a detector matrix, a method for the manufacture of a test element, and to a method for detecting an analyte in a sample, comprising contacting a detector matrix according to the invention with a sample suspected to comprise said analyte. ## Claims ### Claim 1 A covering layer matrix, comprising: a polymeric film former and up-converting phosphor particles; wherein the covering layer matrix is adapted for use as a reflecting layer in a two- or more layer test element; a strongly light-scattering agent; wherein the covering layer matrix lacks an indicator reagent; and wherein the covering layer matrix is configured to cover a detector matrix having the indicator reagent distributed within the detector matrix. ### Claim 2 (depends on 1) The covering layer matrix of claim 1, wherein the covering layer matrix is a matrix adapted for physically contacting an analyte detector matrix. ### Claim 3 (depends on 2) The covering layer matrix of claim 2, wherein the covering layer matrix is a matrix adapted for forming the second layer of the two- or more-layer test element. ### Claim 4 (depends on 1) The covering layer matrix of claim 1, wherein the up-converting phosphor particles are UV-emitting up-converting phosphor particles. ### Claim 5 (depends on 1) The covering layer matrix of claim 1, wherein the polymeric film former is or comprises polymer particles insoluble in a carrier liquid. ### Claim 6 (depends on 5) The covering layer matrix of claim 5, wherein the polymeric film former is selected from the group consisting of polyvinyl esters, polyvinyl acetates, polyacryl esters, polymethacrylic acids, polyvinylamides, polyamides and polystyrene. ### Claim 7 (depends on 1) The covering layer matrix of claim 1, wherein the covering layer matrix further comprises a filling agent. ### Claim 8 (depends on 7) The covering layer matrix of claim 7, wherein the filling agent is SiO2, a silicate, or an aluminum-silicate. ### Claim 9 (depends on 1) The covering layer matrix of claim 1, wherein the strongly light-scattering agent has a refractive index of at least 2. ### Claim 10 (depends on 1) The covering layer matrix of claim 1, wherein the strongly light-scattering agent is a metal oxide, including mixed transition metal oxides. ### Claim 11 (depends on 1) The covering layer matrix of claim 1, wherein the strongly light-scattering agent is TiO2. ### Claim 12 (depends on 11) The covering layer matrix of claim 11, wherein the strongly light-scattering agent has an average particle diameter of 0.2 μm to 0.8 μm. ### Claim 13 (depends on 1) The covering layer matrix of claim 1, wherein the covering layer matrix is adapted for use as an illumination layer. ### Claim 14 (depends on 1) The covering layer matrix of claim 1, wherein the up-converting phosphor particles are coated with one or more inert and/or hydrophilic coatings. ### Claim 15 (depends on 1) A two- or more layer test element comprising a covering layer matrix according to claim 1 and an analyte detector matrix. ### Claim 16 (depends on 15) The test element of claim 15, wherein the analyte detector matrix is the first layer and the covering layer matrix comprising the up-converting phosphor particles is the second layer. ### Claim 17 (depends on 15) The test element of claim 15, wherein the test element is adapted for a measurement setup in which excitation irradiation is supplied from a sample application side of the test element, and wherein detection of emission is performed on the opposite side of the test element. ### Claim 18 (depends on 15) The test element of claim 15, wherein the test element is a test strip, a test tape, or a test disc. ### Claim 19 (depends on 15) The test element according to claim 15, wherein the test element is a glucose test element.