# US 11,029,241 B2 · Enhanced detection through parsing records into signal components > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,029,241 B2 - Document ID: US11029241B2 - Status: Granted - Kind: B2 - Application number: 14/622,789 - Filed: 2015-02-13 - Published: 2016-08-18 - Granted: 2021-06-08 - Earliest claimed: 2015-02-13 - Assignees: Artium Technologies, Inc. - Applicants: Artium Technologies, Inc. - Inventors: William D. Bachalo; Gregory A. Payne; Khalid Ibrahim; Michael J. Fidrich; Chad M. Sipperley - Web page: https://patentgrep.com/patent/US11029241B2 ## CPC - G01N 15/1429: Signal processing - G01N 15/0205: by optical means - G01N 15/0211: {Investigating a scatter or diffraction pattern} - G01N 15/1434: Optical arrangements - G01N 2015/1027: {Determining speed or velocity of a particle} - G01N 2015/145: {by pattern of light, e.g. fringe pattern} - G01N 2015/1461: {Coincidence detecting; Circuits therefor} - G01N 2015/1493: {Particle size} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Methods and apparatuses to detect particles in dense particle fields are described. A time varying signal is partitioned into a plurality of segments. Parameters are determined from the segments. The time varying signal is parsed into a plurality of individual particle signal components based on the plurality of parameters. ## Claims ### Claim 1 A method to enhance detection of particles, comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) determining a plurality of parameters from the plurality of timing segments, wherein the plurality of parameters comprise at least one of a frequency and a phase; d) determining a change in the at least one of the frequency and the phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events; and g) adjusting the plurality of timing segments, wherein the partitioning and determining operations are repeated iteratively for the adjusted segments until at least the first individual particle signal component and the second individual particle signal component are identified, wherein operations a), b), c), d), e), f), and g) are performed by a processor. ### Claim 2 (depends on 1) The method of claim 1, wherein the plurality of parameters further comprise: an amplitude, a signal duration, a transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 3 (depends on 1) The method of claim 1, further comprising: locating peak amplitudes in the time varying signal; and adjusting the plurality of timing segments based on the located peak amplitudes to capture durations of at least the first individual particle signal component and the second individual particle signal component. ### Claim 4 (depends on 1) The method of claim 1, further comprising: determining at least one of the plurality of parameters for each of the first individual particle signal component and the second individual particle signal component; validating each of the first individual particle signal component and the second individual particle signal component based on the at least one of the plurality of parameters; and determining at least one of a velocity and a size of a particle from each of the validated individual particle signal components. ### Claim 5 (depends on 1) The method of claim 1, wherein the time varying signal is a sampled signal. ### Claim 6 A method to mitigate particle measurement errors in a dense particle field, comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) locating peak amplitudes in the plurality of timing segments; d) determining a change in at least one of parameters comprising a frequency and a phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident event; and g) determining an estimated particle transit time, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the estimated transit time wherein operations a, b, c, d, e, f, and g are performed by a processor. ### Claim 7 (depends on 6) The method of claim 6, wherein the at least one of parameters further comprise a signal duration, a particle transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 8 (depends on 6) The method of claim 6, further comprising: validating each of the first individual particle signal component and the second individual particle signal component based on the at least one of the frequency and the phase; and determining at least one of a velocity and a size of a particle from each of the validated first individual particle signal component and the second individual particle signal component. ### Claim 9 (depends on 6) The method of claim 6, wherein the time varying signal comprises a number of samples based on at least one of a signal duration and a sampling frequency. ### Claim 10 A method to mitigate particle measurement errors in a dense particle field, comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a continuous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) locating peak amplitudes in the plurality of timing segments; d) determining a change in at least one of parameters comprising a frequency and a phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events; and g) determining a duration of the time varying signal, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the duration wherein operations a, b, c, d, e, f, and g are performed by a processor. ### Claim 11 A method to mitigate particle measurement errors in a dense particle field, comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) locating peak amplitudes in the plurality of timing segments; d) determining a change in at least one of parameters comprising a frequency and a phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events; and g) adjusting a duration of the plurality of timing segments based on the peak amplitudes, wherein operations b, c and d are repeated iteratively for the adjusted timing segments until at least the first individual particle signal component and the second individual particle signal component are identified, wherein operations a, b, c, d, e, f, and g are performed by a processor. ### Claim 12 A non-transitory machine-readable medium storing data to cause a data processing system to perform a method to enhance detection of particles comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) determining a plurality of parameters from the plurality of timing segments, wherein the plurality of parameters comprise at least one of a frequency, and a phase; d) determining a change in the at least one of the frequency and the phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; and f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events. ### Claim 13 (depends on 12) The non-transitory machine-readable medium of claim 12, further comprising data that cause the data processing system to perform operations comprising: adjusting the plurality of timing segments, wherein the partitioning and determining operations are repeated iteratively for the adjusted segments until at least the first individual particle signal component and the second individual particle signal component are identified. ### Claim 14 (depends on 12) The non-transitory machine-readable medium of claim 12, wherein the plurality of parameters further comprise an amplitude, a signal duration, a transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 15 (depends on 12) The non-transitory machine-readable medium of claim 12, further comprising data that cause the data processing system to perform operations comprising: locating peak amplitudes in the time varying signal; and adjusting the plurality of timing segments based on the located peak amplitudes to capture durations of at least the first individual particle signal component and the second individual particle signal component. ### Claim 16 (depends on 12) The non-transitory machine-readable medium of claim 12, further comprising data that cause the data processing system to perform operations comprising: determining at least one of the plurality of parameters for each of the first individual particle signal component and the second individual particle signal component; validating each of the first individual particle signal component and the second individual particle signal component based on the at least one of the plurality of parameters; and determining at least one of a velocity and a size of a particle from each of the validated first individual particle signal component and the second individual particle signal component. ### Claim 17 (depends on 12) The non-transitory machine-readable medium of claim 12, wherein the time varying signal is a digitized signal, wherein a number of samples of the digitized signal is determined based on at least one of a signal duration and a sampling frequency. ### Claim 18 A non-transitory machine-readable medium storing data to cause a data processing system to perform a method to mitigate particle measurement errors in a dense particle field, comprising: a) receiving a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; b) partitioning the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; c) locating peak amplitudes in the plurality of timing segments; d) determining a change in at least one of parameters comprising a frequency and a phase between the first timing segment and the second timing segment; e) identifying the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; and f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events, wherein operations a, b, c, d, e, and f are performed by a processor. ### Claim 19 (depends on 18) The non-transitory machine-readable medium of claim 18, wherein the at least one of parameters further comprise a signal duration, a particle transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 20 (depends on 18) The non-transitory machine-readable medium of claim 18, further comprising data that cause the data processing system to perform operations comprising: determining an estimated particle transit time, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the estimated transit time. ### Claim 21 (depends on 18) The non-transitory machine-readable medium of claim 18, further comprising data that cause the data processing system to perform operations comprising: determining a duration of the time varying signal, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the duration. ### Claim 22 (depends on 18) The non-transitory machine-readable medium of claim 18, further comprising data that cause the data processing system to perform operations comprising: validating each of the first individual particle signal component and the second individual particle signal component based on the at least one of the frequency and the phase; and determining at least one of a velocity and a size of a particle from each of the validated first individual particle signal component and the second individual particle signal component. ### Claim 23 (depends on 18) The non-transitory machine-readable medium of claim 18, wherein the time varying signal comprises a number of samples based on at least one of a signal duration and a sampling frequency. ### Claim 24 (depends on 18) The non-transitory machine-readable medium of claim 18, further comprising data that cause the data processing system to perform operations comprising: adjusting a duration of the plurality of timing segments based on the peak amplitudes, wherein operations b, c and d are repeated iteratively for the adjusted timing segments until at least the first individual particle signal component and the second individual particle signal component are identified. ### Claim 25 An apparatus to enhance detection of particles, comprising: a memory, and a processor coupled to the memory, wherein the processor is to a) receive a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal; the processor is to b) partition the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment, the processor is to c) determine a plurality of parameters from the plurality of timing segments, wherein the plurality of parameters comprise at least one of a frequency and a phase; the processor is to d) determine a change in at least one of the frequency and the phase between the first timing segment and the second timing segment; the processor is to e) identify the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; and the processor is to f) identifying the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events. ### Claim 26 (depends on 25) The apparatus of claim 25, wherein the processor is further to adjust the plurality of timing segments, wherein the partitioning and determining operations are repeated iteratively for the adjusted segments until at least the first individual particle signal component and the second individual particle signal component are identified. ### Claim 27 (depends on 25) The apparatus of claim 25, wherein the plurality of parameters further comprises an amplitude, a signal duration, a transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 28 (depends on 25) The apparatus of claim 25, wherein the processor is further to locate peak amplitudes in the time varying signal; and to adjust the plurality of timing segments based on the located peak amplitudes to capture durations of the first individual particle signal component and the second individual particle signal component. ### Claim 29 (depends on 25) The apparatus of claim 25, wherein the processor is further to determine at least one of the plurality of parameters for each of the first individual particle signal component and the second individual particle signal component; the processor is further to validate each of the first individual particle signal component and the second individual particle signal component based on the at least one of the plurality of parameters; and the processor is further to determine at least one of a velocity and a size of a particle from each of the validated first individual particle signal component and the second individual particle signal component. ### Claim 30 (depends on 25) The apparatus of claim 25, wherein the time varying signal is a sampled signal. ### Claim 31 An apparatus to mitigate particle measurement errors in a dense particle field, comprising: a memory; and a processor coupled to the memory, wherein the processor is to a) receive a time varying signal generated from a light scattered from one or more particles that forms an interference fringe pattern, wherein the time varying signal is a contiguous signal, the processor is to b) partition the time varying signal into a plurality of timing segments comprising a first timing segment and a second timing segment; wherein the processor is to c) locate peak amplitudes in the plurality of timing segments; wherein the processor is to d) determine a change in at least one of parameters comprising a frequency and a phase between the first timing segment and the second timing segment; wherein the processor is to e) identify the first timing segment and the second timing segment as being associated with a single particle, when the change is not greater than a threshold; and wherein the processor is to f) identify the first timing segment as a first individual particle signal component produced by a first particle and identifying the second timing segment as a second individual particle signal component produced by a second particle, when the change in the at least one of the frequency and the phase is greater than the threshold when a sample volume has a plurality of particles including the first particle and the second particle within a time duration to mitigate at least one of a particle size measurement error and a particle velocity measurement error and a false counting of particles under coincident events. ### Claim 32 (depends on 31) The apparatus of claim 31, wherein the at least one of parameters further comprise a signal duration, a particle transit time, a signal-to-noise ratio, or any combination thereof. ### Claim 33 (depends on 31) The apparatus of claim 31, wherein the processor is further to determine an estimated particle transit time, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the estimated transit time. ### Claim 34 (depends on 31) The apparatus of claim 31, wherein the processor is further to determine a duration of the time varying signal, wherein at least the first individual particle signal component and the second individual particle signal component are identified based on the duration. ### Claim 35 (depends on 31) The apparatus of claim 31, wherein the processor is further to validate each of the first individual particle signal component and the second individual particle signal component based on the at least one of the frequency and the phase; and wherein the processor is further to determine at least one of a velocity and a size of a particle from each of the validated first individual particle signal component and the second individual particle signal component. ### Claim 36 (depends on 31) The apparatus of claim 31, wherein the time varying signal is a sampled signal. ### Claim 37 (depends on 31) The apparatus of claim 31, wherein the processor is further to adjust a duration of the plurality of timing segments based on the peak amplitudes, wherein operations b, c and d are repeated iteratively for the adjusted timing segments until at least the first individual particle signal component and the second individual particle signal component are identified.