# US 11,029,208 B2 · Microspectroscope having automatic sample detection function > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,029,208 B2 - Document ID: US11029208B2 - Status: Granted - Kind: B2 - Application number: 16/783,294 - Filed: 2020-02-06 - Published: 2020-08-27 - Granted: 2021-06-08 - Earliest claimed: 2019-02-15 - Assignees: Jasco Corporation - Applicants: JASCO Corporation - Inventors: Kento Aizawa; Yoshiko Kubo; Norihito Fujiwara; Katsunori Morii - Web page: https://patentgrep.com/patent/US11029208B2 ## CPC - G01J 3/44: Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} - G01J 3/0256: {Compact construction} - G01J 3/2823: {Imaging spectrometer} - G01N 21/65: Raman scattering - G06T 5/50: using two or more images, e.g. averaging or subtraction - G06T 2207/10056: Microscopic image - G06T 2207/20221: Image fusion; Image merging - G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL ## Abstract The present invention relates to improvement in accuracy of an automatic sample detection technique in spectrometry of a microspectroscope. A microspectroscope 10 comprises: a light source 12 that emits an excitation light to a sample 20; a condensing lens 16 that emits the excitation light to a predetermined position of the sample 20 and condenses a reflected light or a transmitted light from the sample 20; a spectrometer 24 that detects a condensed light; and an analysis control unit 30 for analyzing a signal from the spectrometer 24; the microspectroscope 10 that uses an observation image of the sample 20 to perform spectrometry, wherein the analysis control unit 30 comprises: an image storage part 32 that converts the observation image to an all-in-focus image to store the all-in-focus image; and a control part 34 that makes the microspectroscope 10 to perform measurement, the image storage part 32 adds a detected information of a sample point that is a target of spectrometry to the all-in-focus image to create a sample search image data, and the control part 34 automatically detects the sample point by using the sample search image data. ## Claims ### Claim 1 A microspectroscope comprising: a light source that emits an excitation light to a sample; a condensing lens that emits the excitation light to a predetermined position of the sample and condenses a reflected light or a transmitted light from the sample; a spectrometer that detects a condensed light; and an analysis control unit that analyzes a signal from the spectrometer, the microspectroscope that performs spectrometry by using an observation image of the sample, wherein: the analysis control unit comprises an image storage part that converts the observation image to an all-in-focus image to store the same, and a control part that makes the microspectroscope to perform measurement, the image storage part adds a detected information of a sample point that is a target of spectrometry to the all-in-focus image to create a sample search image data, and the control part automatically detects the sample point by using the sample search image data. ### Claim 2 (depends on 1) The microspectroscope according to claim 1, wherein the sample search image data is added with any of a center of gravity, an area, a circularity, color, a Feret's diameter, and an angle of rotation of the sample point as the detected information to the sample search image by analyzing the all-in-focus image data. ### Claim 3 (depends on 1, 2) The microspectroscope according to claim 1 or 2, wherein a height position information of the sample point in the sample is added as the detected information to the sample search image data. ### Claim 4 (depends on 1) The microspectroscope according to claim 1, wherein the image storage part performs a binarization processing to the all-in-focus image as a preliminary processing for creating the sample search image data. ### Claim 5 (depends on 1) The microspectroscope according to claim 1, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 6 (depends on 2) The microspectroscope according to claim 2, wherein a height position information of the sample point in the sample is added as the detected information to the sample search image data. ### Claim 7 (depends on 2) The microspectroscope according to claim 2, wherein the image storage part performs a binarization processing to the all-in-focus image as a preliminary processing for creating the sample search image data. ### Claim 8 (depends on 3) The microspectroscope according to claim 3, wherein the image storage part performs a binarization processing to the all-in-focus image as a preliminary processing for creating the sample search image data. ### Claim 9 (depends on 6) The microspectroscope according to claim 6, wherein the image storage part performs a binarization processing to the all-in-focus image as a preliminary processing for creating the sample search image data. ### Claim 10 (depends on 2) The microspectroscope according to claim 2, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 11 (depends on 3) The microspectroscope according to claim 3, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 12 (depends on 4) The microspectroscope according to claim 4, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 13 (depends on 6) The microspectroscope according to claim 6, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 14 (depends on 7) The microspectroscope according to claim 7, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 15 (depends on 8) The microspectroscope according to claim 8, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image. ### Claim 16 (depends on 9) The microspectroscope according to claim 9, wherein the control part uses the sample search image data to automatically detect the sample point by a template matching as a matching action of the image.