# US 11,028,453 B2 · QTLs associated with and methods for identifying lodging resistance in soybean > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,028,453 B2 - Document ID: US11028453B2 - Status: Granted - Kind: B2 - Application number: 16/061,046 - Filed: 2017-02-10 - Published: 2019-01-24 - Granted: 2021-06-08 - Earliest claimed: 2016-02-11 - Assignees: Pioneer Hi-Bred International, Inc. - Applicants: Pioneer Hi-Bred International, Inc. - Inventors: Sarah Atwood; Laura Jean Wolf; Leslie Charles Kuhlman; Donald Earl Kyle; Daniel Lewis Thomas; John Bryan Woodward; Ming Yang - Web page: https://patentgrep.com/patent/US11028453B2 ## CPC - C12Q 1/6895: for plants, fungi or algae - A01H 1/045: {using molecular markers} - A01H 5/10: Seeds - A01H 6/542: {Glycine max [soybean]} - C07K 14/415: from plants - C12Q 1/686: Polymerase chain reaction [PCR] - C12Q 2600/13: Plant traits - C12Q 2600/156: Polymorphic or mutational markers - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The disclosure relates to methods and compositions for identifying and/or selecting soybean plants that have resistance to lodging, have improved resistance to lodging, or are susceptible to lodging. The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Also provided are soybean plants that display resistance or improved resistance to lodging that are generated by the methods described herein. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure. ## Claims ### Claim 1 A method of selecting a first soybean plant or soybean germplasm that displays improved lodging resistance, the method comprising: (a) detecting in a first soybean plant or soybean germplasm at least one allele of one or more marker locus within or linked to a quantitative trait locus (QTL) associated with improved lodging resistance, wherein the allele positively correlates with improved lodging resistance, and wherein the at least one allele of the one or more marker locus comprises an allele selected from the group consisting of allele A of marker locus Gm13:36431456, allele T of marker locus Gm13:36490271, allele T of marker locus Gm13:36491753, allele T of marker locus Gm13:36491754, allele T of marker locus Gm13:36492037, allele G of marker locus Gm13:36492926, allele A of marker locus Gm13:36492955, allele G of marker locus Gm13:36493615, allele G of marker locus Gm13:36494839, allele A of marker locus Gm13:36517239, allele T of marker locus Gm13:36539789, allele T of marker locus Gm13:36539798, allele C of marker locus Gm13:36540415, allele C of marker locus Gm13:36593549, allele T of marker locus Gm13:36613902, allele T of marker locus Gm13:36644196, allele T of marker locus Gm13:36644203, allele T of marker locus Gm13:36644207, allele A of marker locus Gm13:36678427, allele G of marker locus Gm13:36697528, allele T of marker locus Gm13:36795108, allele C of marker locus Gm13:36704369, allele A of marker locus Gm13:36300296, allele T of marker locus Gm13:36567042, allele A of marker locus Gm13:36792347, allele A of marker locus Gm13:36864280, and allele G of marker locus Gm13:37443784; (b) selecting a first soybean plant or soybean germplasm comprising the at least one allele from step (a), thereby selecting a soybean plant or soybean germplasm that displays improved lodging resistance; and (c) crossing the selected first soybean plant or soybean germplasm with a second soybean plant or soybean germplasm to introgress the QTL into a progeny soybean plant or soybean germplasm. ### Claim 2 (depends on 1) The method of claim 1, wherein the at least one allele comprises allele C of marker locus Gm13:36593549. ### Claim 3 (depends on 1) The method of claim 1, wherein detecting comprises amplifying a nucleic acid sequence comprising the marker locus of each allele and detecting the resulting amplified nucleic acid comprising each marker locus. ### Claim 4 (depends on 3) The method of claim 3, wherein amplifying comprises amplification of at least a portion of one or more genomic regions of the soybean genome selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, and 27. ### Claim 5 (depends on 4) The method of claim 4, wherein the wherein the amplification comprises providing one or more nucleic acid primers, wherein the nucleic acid primers comprise the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 32, 33, 36, 37, 40, 41, 44, 45, 48, 49, 52, 53, 56, and 57. ### Claim 6 (depends on 5) The method of claim 5, wherein the one or more nucleic acid primers comprise a detectable label. ### Claim 7 (depends on 1) The method of claim 1, wherein detecting comprises hybridization with one or more nucleic acid probes, wherein the nucleic acid probes comprise the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 34, 38, 42, 46, 50, 54, and 58. ### Claim 8 (depends on 7) The method of claim 7, wherein the one or more nucleic acid probes comprise a detectable label. ### Claim 9 (depends on 1) The method of claim 1, wherein the marker locus is further associated with decreased height of the plant. ### Claim 10 (depends on 1) The method of claim 1, wherein the second soybean plant or soybean germplasm displays decreased lodging resistance as compared to the first soybean plant or soybean germplasm, and wherein the introgressed soybean plant or soybean germ plasm displays an improved lodging resistance as compared to the second soybean plant or soybean germ plasm. ### Claim 11 (depends on 1) The method of claim 1, wherein the second soybean plant or soybean germ plasm displays increased height as compared to the first soybean plant or soybean germ plasm, and wherein the introgressed soybean plant or soybean germ plasm displays decreased height as compared to the second soybean plant or soybean germplasm. ### Claim 12 (depends on 1) The method of claim 1, further comprising (d) analyzing progeny soybean germplasm to determine the presence of improved lodging resistance; and (e) selecting progeny soybean germplasm that test positive for the presence of improved lodging resistance as being soybean germplasm into which germplasm having said QTL has been introgressed. ### Claim 13 (depends on 12) The method of claim 12, wherein the analyzing progeny soybean germ plasm further comprises determining the presence of decreased height.