Covalent tethering of functional groups to proteins
AssigneePromega Corporation
InventorsKeith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
Life of this patent
Estimating the term…
Abstract
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
Claims20
A compound of formula (I):
- R-linker-A-X,
- wherein R is selected from the group consisting of a nucleic acid molecule, an amino acid, a peptide, a receptor protein, a glycoprotein, an antibody, a lipid, a hapten, a receptor ligand, a fluorophore, a drug, a toxin, and an affinity molecule,
- wherein R is not biotin,
- wherein the linker is a multiatom straight or branched chain including C, N, S and/or O and comprising no more than 30 carbon atoms,
- wherein A-X is a substrate for a dehalogenase,
- wherein A is (CH2)n and n=2-10, and
- wherein X is a halogen.
A compound of formula (I):
- R-linker-A-X, wherein R is selected from the group consisting of a nucleic acid molecule, an amino acid, a peptide, a receptor protein, a glycoprotein, an antibody, a lipid, a hapten, a receptor ligand, a fluorophore, and a toxin, wherein the linker is a multiatom straight or branched chain including C, N, S and/or O and comprising no more than 30 carbon atoms, wherein A-X is a substrate for a dehalogenase, wherein A is (CH2)n and n=2-10, and wherein X is a halogen.
Dependencies are inferred from the claim text.