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US 11,028,419 B2Granted
Biosynthesis of human milk oligosaccharides in engineered bacteria
AssigneeGlycosyn LLC
InventorsMassimo Merighi, John M. McCoy, Matthew Ian Heidtman
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Abstract
The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Claims21
A method for producing a fucosylated oligosaccharide in an E.coli bacterium, comprising
- (a) providing an E.coli bacterium comprising a β-galactosidase activity between 0.05 and 5 units and a mutation in a lacA gene, wherein said E.coli bacterium further comprises an exogenous lactose-accepting fucosyltransferase gene;
- (b) culturing said E.coli bacterium in the presence of lactose;
- and
- (c) retrieving a fucosylated oligosaccharide from said E.coli bacterium or from a culture supernatant of said E.coli bacterium,
- wherein a functional β-galactosidase gene is inserted into an endogenous gene of said E. coli bacterium.
2 more elements
Dependencies are inferred from the claim text.