Back to results
US 11,028,416 B2Granted
Engineered biosynthetic pathways for production of tyramine by fermentation
AssigneeZymergen Inc.
InventorsMichael Shareef Siddiqui, Stefan de Kok, Alexander Glennon Shearer, Franklin Lu, Cara Ann Tracewell, Steven M. Edgar
Life of this patent
Estimating the term…
Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods.
Claims9
An engineered Yarrowia lipolytica or Corynebacterium glutamicum cell, wherein the engineered cell expresses:
- (a) a heterologous tyrosine decarboxylase (TYDC) having at least 95% amino acid sequence identity to a pyridoxal-dependent decarboxylase (TYDC) from Enterococcus faecium Com15 comprising SEQ ID NO:1;
- and
- (b) a heterologous, 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase having at least 95% amino acid sequence identity to a wild-type phospho-2-dehydro-3-deoxyheptonate aldolase (DAHP synthase) from Saccharomyces cerevisiae S288c comprising SEQ ID NO:9;
- wherein, when cultured, the engineered Yarrowia lipolytica or Corynebacterium glutamicum cell produces tyramine at a level greater than 50 mg/L of culture medium.
1 more element
Dependencies are inferred from the claim text.