# US 11,028,406 B2 · Podosphaera leucotricha resistance providing genes in Malus domestica

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- Number: US 11,028,406 B2
- Document ID: US11028406B2
- Status: Granted
- Kind: B2
- Application number: 15/765,752
- Filed: 2016-10-05
- Published: 2018-10-18
- Granted: 2021-06-08
- Earliest claimed: 2015-10-07
- Assignees: Fondazione Edmund Mach
- Applicants: Fondazione Edmund Mach
- Inventors: Mickael Malnoy; Stefano Pessina; Riccardo Velasco; Dario Angeli; H. J. Schouten
- Web page: https://patentgrep.com/patent/US11028406B2

## CPC

- C12N 15/8282: {for fungal resistance}
- C07K 14/415: from plants
- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Provided herein are Podosphaera leucotricha resistance conferring genes, plants, plant parts and seeds comprising the present resistance providing genes and the use thereof for selecting Podosphaera leucotricha resistant plants. Specifically, the present invention relates to Podosphaera leucotricha resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring genes is the primary amino acid sequence represented SEQ ID No. 1, or a primary amino acid sequence with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity with SEQ ID No. 1; and wherein said resistance conferring gene is impaired.

## Claims

### Claim 1

A Malus domestica plant which is resistant to Podosphaera leucotricha comprising a non-natural modification introduced into its genome that results in:
reduced transcription of a nucleic acid sequence of SEQ ID NO: 2 as compared to a Malus domestica plant that is not resistant to Podosphaera leucotricha,
wherein the non-natural modification is in the nucleic acid sequence of SEQ ID NO. 2.

### Claim 2 (depends on 1)

The Malus domestica plant of claim 1 wherein the non-natural modification results in gene silencing.

### Claim 3 (depends on 1)

A seed, tissue, or plant part of the Malus domestica plant of claim 1, wherein the seed, tissue, or plant part comprises the modification that results in:
reduced transcription of the nucleic acid sequence of SEQ ID NO: 2 as compared to a Malus domestica plant that is not resistant to Podosphaera leucotricha.

### Claim 4

A method for obtaining a Malus domestica plant that is resistant to Podosphaera leucotricha, the method comprising modifying the Malus domestica plant by: reducing transcription of a nucleic acid sequence of SEQ ID NO: 2 as compared to a Malus domestica plant that is not resistant to Podosphaera leucotricha, wherein the reduction results from a non-natural modification in the nucleic acid sequence of SEQ ID NO. 2.

### Claim 5 (depends on 4)

The method of claim 4, wherein the nucleic acid sequence of SEQ ID NO: 2 is silenced.

### Claim 6 (depends on 4)

A Malus domestica plant produced from the method according to claim 4, wherein the plant has:
reduced transcription of the nucleic acid sequence of SEQ ID NO: 2 as compared to a Malus domestica plant that is not resistant to Podosphaera leucotricha.

### Claim 7 (depends on 6)

A seed, tissue, or plant part of the Malus domestica plant according to claim 6, wherein the seed, tissue, or plant part has:
reduced transcription of the nucleic acid sequence of SEQ ID NO: 2 as compared to a Malus domestica plant that is not resistant to Podosphaera leucotricha.