# US 11,028,403 B2 · Methods of increasing plant biomass and oilseed production > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,028,403 B2 - Document ID: US11028403B2 - Status: Granted - Kind: B2 - Application number: 15/569,230 - Filed: 2016-04-28 - Published: 2018-11-01 - Granted: 2021-06-08 - Earliest claimed: 2015-04-28 - Assignees: University of Guelph - Applicants: University of Guelph - Inventors: Michael Emes; Ian Tetlow; Fushan Liu - Web page: https://patentgrep.com/patent/US11028403B2 ## CPC - C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis} - C12N 9/107: {1,4-Alpha-glucan branching enzyme (2.4.1.18)} - C12N 15/52: Genes encoding for enzymes or proenzymes - C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]} - C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition} - C12N 15/8261: {with agronomic (input) traits, e.g. crop yield} - C12P 7/64: Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats - C12P 19/00: Preparation of compounds containing saccharide radicals (ketoaldonic acids C12P7/58) - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - Y02E 50/10: Biofuels, e.g. bio-diesel - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE - Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION ## Abstract A novel method for increasing plant biomass and oilseed production is described. Transgenic plants with increased biomass and oilseed production are also described. ## Claims ### Claim 1 A transgenic plant produced using a method comprising: a. contacting a plant cell with a nucleic acid molecule encoding an exogenous starch-branching enzyme selected from starch-branching enzyme 1 (SBEI) and starch-branching enzyme 2b (SBEIIb) to obtain a transformed plant cell, b. expressing the nucleic acid molecule in the transformed plant cell, and c. producing a plant from the transformed plant cell, wherein the nucleic acid molecule is from a cereal plant and wherein the plant is a dicotyledon, wherein the plant has increased biomass, seed production and/or oil production compared to a wild-type plant not transformed with the nucleic acid molecule, wherein the nucleic acid molecule comprises a nucleic acid sequence that has at least 80% sequence identity with a nucleic acid sequence selected from SEQ ID NO: 1 or 3, and wherein the cereal plant is maize, wheat, barley, sorghum, oat, rye, millet, triticale or rice. ### Claim 2 (depends on 1) A transgenic seed of the plant of claim 1. ### Claim 3 (depends on 1) A transgenic plant cell of the plant of claim 1. ### Claim 4 A transformed plant cell, plant or plant part expressing at least one nucleic acid molecule encoding an exogenous starch-branching enzyme selected from starch-branching enzyme 1 (SBEI) and starch-branching enzyme 2b (SBEIIb), wherein the nucleic acid molecule is from a cereal plant and the transformed plant cell, plant or plant part is a dicotyledon and wherein said transformed plant or plant part or a plant or plant part obtained from said transformed plant cell has increased biomass, seed production and/or oil production compared to a wild-type plant or plant part not transformed with the nucleic acid molecule, wherein the nucleic acid molecule comprises a nucleic acid sequence that has at least 80% sequence identity with a nucleic acid sequence selected from SEQ ID NO: 1 or 3, and wherein the cereal plant is maize, wheat, barley, sorghum, oat, rye, millet, triticale or rice. ### Claim 5 (depends on 4) The transformed plant cell, plant or plant part of claim 4, wherein the activity or expression of at least one endogenous starch-branching enzyme in the transformed plant cell, plant or plant part is reduced or eliminated independently from expression of the nucleic acid molecule. ### Claim 6 (depends on 4) The transformed plant cell, plant or plant part of claim 4, wherein the transformed plant cell, plant or plant part is an oil-seed crop. ### Claim 7 (depends on 4) The transformed plant cell, plant or plant part of claim 4 expressing a first nucleic acid molecule encoding a first exogenous starch-branching enzyme and a second nucleic acid molecule encoding a second exogenous starch-branching enzyme. ### Claim 8 (depends on 7) The transformed plant cell, plant or plant part of claim 7, wherein the first nucleic acid molecule comprises a nucleic acid sequence that has at least 80% sequence identity with SEQ ID NO: 1, and the second nucleic acid molecule comprises a nucleic acid sequence that has at least 80% sequence identity with SEQ ID NO: 3. ### Claim 9 (depends on 6) The transformed plant cell, plant or plant part of claim 6, wherein the oil-seed crop is canola, camelina, cotton, flaxseed, mustard, rapeseed, soybean, sunflower seed or palm. ### Claim 10 (depends on 1) The transgenic plant of claim 1, wherein the method further comprises the step of reducing the activity or expression of at least one endogenous starch- branching enzyme in the plant cell.