# US 11,028,396 B2 · DNA aptamer specifically binding to CFP10, and use thereof

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- Number: US 11,028,396 B2
- Document ID: US11028396B2
- Status: Granted
- Kind: B2
- Application number: 16/649,741
- Filed: 2018-09-19
- Published: 2020-10-15
- Granted: 2021-06-08
- Earliest claimed: 2017-09-25
- Assignees: Md Aptus Inc.
- Applicants: Md Aptus Inc.
- Inventors: Yoon-Keun Kim; Changill Ban; Chulhun Chang
- Web page: https://patentgrep.com/patent/US11028396B2

## CPC

- C12N 15/115: Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith {; Nucleic acids binding to non-nucleic acids, e.g. aptamers}
- G01N 33/553: Metal or metal coated
- G01N 33/5695: {Mycobacteria}
- G01N 2800/26: Infectious diseases, e.g. generalised sepsis
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to: a DNA aptamer specifically binding to culture filtrate 10 kDa (CFP10); a biosensor for diagnosing tuberculosis, comprising the same: and an information providing method for diagnosing tuberculosis. The present applicants have ascertained that the DNA aptamer, according to the present invention, has the ability to specifically bind to a CFP10 protein and that the binding strength thereof is strong. When the DNA aptamer of the present invention is used, excellent stability superior to that of ELISA methods using existing antibodies can be expected, and thus it is expected that the aptamer can be effectively used in the development of a composition for diagnosing tuberculosis, a biosensor for diagnosing tuberculosis, an information providing method for diagnosing tuberculosis, and the like.

## Claims

### Claim 1

A DNA aptamer that specifically binds to culture filtrate protein 10 kDa (CFP10), wherein the DNA aptamer consists of a nucleotide sequence of SEQ ID NO: 6.

### Claim 2 (depends on 1)

The DNA aptamer of claim 1, wherein the CFP10 is expressed in Mycobacterium tuberculosis H37Rv.

### Claim 3 (depends on 1)

A composition for diagnosing tuberculosis, the composition comprising the DNA aptamer of claim 1.

### Claim 4

A biosensor for diagnosing tuberculosis, the biosensor comprising: a DNA aptamer specific to culture filtrate protein 10 kDa (CFP10); and a board on which the DNA aptamer is immobilized, wherein the DNA aptamer consists of a nucleotide sequence of SEQ ID NO: 6.

### Claim 5 (depends on 4)

The biosensor of claim 4, wherein the board consists of a metal electrode layer and a metal nanoparticle layer.

### Claim 6 (depends on 5)

The biosensor of claim 5, wherein the metal comprises gold (Au).

### Claim 7 (depends on 4)

A method of providing information for diagnosing tuberculosis, the method comprising:
(1) treating a subject sample to the biosensor of claim 4; and
(2) measuring a level of culture filtrate protein 10 kDa (CFP10) bound to the biosensor.

### Claim 8 (depends on 1)

A method of diagnosing tuberculosis, the method comprising bringing the DNA aptamer of claim 1 into contact with a subject sample.

### Claim 9 (depends on 4)

A method of diagnosing tuberculosis, the method comprising: treating a subject sample to the biosensor of claim 4; and measuring a level of culture filtrate protein 10 kDa (CFP10) bound to the biosensor.