# US 11,028,376 B2 · DNA polymerases from the red sea brine pool

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- Number: US 11,028,376 B2
- Document ID: US11028376B2
- Status: Granted
- Kind: B2
- Application number: 16/591,832
- Filed: 2019-10-03
- Published: 2020-01-23
- Granted: 2021-06-08
- Earliest claimed: 2014-04-11
- Assignees: King Abdullah University of Science and Technology
- Applicants: King Abdullah University of Science and Technology
- Inventors: Samir M. Hamdan; Masateru Takahashi
- Web page: https://patentgrep.com/patent/US11028376B2

## CPC

- C12N 9/1252: {DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase}
- C12P 19/34: Polynucleotides, e.g. nucleic acids, oligoribonucleotides
- C12Y 207/07007: DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES

## Abstract

A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.

## Claims

### Claim 1

A composition for amplifying nucleic acids comprising:
an isolated DNA polymerase comprising the sequence of SEQ ID NO:3 and an amount of Ethylenediaminetetraacetic acid (EDTA), Tris-HCl and/or dithiothreitol (DTT).

### Claim 2 (depends on 1)

The composition of claim 1, further comprising glycerol.

### Claim 3 (depends on 1)

The composition of claim 1, comprising the sequence of SEQ ID NO: 3 and DTT.

### Claim 4 (depends on 2)

The composition of claim 2, comprising an amount of EDTA, Tris-HCl and DTT.

### Claim 5 (depends on 1)

The composition of claim 1, comprising the sequence of SEQ II) NO: 3 and an amount of EDTA.

### Claim 6

A composition for amplifying nucleic acids comprising:
an isolated DNA polymerase comprising the sequence of SEQ ID NO:3 and 50% glycerol.