# US 11,028,361 B2 · Compositions and methods for stabilizing susceptible compounds

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- Number: US 11,028,361 B2
- Document ID: US11028361B2
- Status: Granted
- Kind: B2
- Application number: 16/801,668
- Filed: 2020-02-26
- Published: 2020-06-18
- Granted: 2021-06-08
- Earliest claimed: 2011-02-07
- Assignees: Life Technologies Corporation
- Applicants: Life Technologies Corporation
- Inventors: Richard Fike; Bruce Branchaud; Shawn Barrett
- Web page: https://patentgrep.com/patent/US11028361B2

## CPC

- C12N 5/0018: {Culture media for cell or tissue culture (media for specific animal cell type C12N5/06)}
- C12N 2500/34: Sugars
- C12N 2500/46: Amines, e.g. putrescine
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Methods for increasing the stability of, or protecting, labile components such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium. Stability of the labile compound is increased either, by derivatization of the labile compound with chemicals, or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule, or by the use of sequestering agents. Encapsulation includes the encapsulation of dendrimers complexes of susceptible compounds within the microcapsule, thereby providing the controlled release of the susceptible compound that was protected. These methods may improve and extend storage conditions of compositions comprising the labile compounds, improve shipping and handling of compositions comprising the labile compounds, such as dry media formulations, at room temperature rather than at lower temperatures thereby decreasing shipping costs. Stabilization of labile compounds in compositions such as dry format media can be viewed as a contribution to green technology.

## Claims

### Claim 1

A method of encapsulating a labile compound for protection from adverse reactions within a composition, comprising:
a. reacting the labile compound with a dendrimer to produce a dendrimer-labile compound complex;
b. encapsulating the dendrimer-labile compound complex of step a) within a sequestering agent, to produce an encapsulated dendrimer-labile compound complex, and;
c. admixing the encapsulated dendrimer-labile compound complex of step b) with one or more components in the composition, wherein said labile compound is ethanolamine.

### Claim 2 (depends on 1)

The method of claim 1, wherein said composition is a cell culture medium, a cell culture supplement, or a cell culture feed.

### Claim 3 (depends on 2)

The method of claim 2, wherein said cell culture medium, cell culture supplement, or cell culture feed is a dry media.

### Claim 4 (depends on 3)

The method of claim 3, wherein said dry media is an agglomerated media.

### Claim 5 (depends on 1)

The method of claim 1, wherein the sequestering agent is a soluble matrix made up of a molecule comprising an alcohol, a ketone or an aldehyde.

### Claim 6 (depends on 5)

The method of claim 5 wherein the soluble matrix is made up of a sugar.

### Claim 7 (depends on 6)

The method of claim 6, wherein the sugar is maltodextrin.

### Claim 8 (depends on 1)

The method of claim 1 wherein the sequestering agent is an insoluble matrix selected from the group consisting of alginate, poly-L-lactic acid, chitosan, agarose, gelatin, hyaluronic acid, chondroitin sulfate, dextran, dextran sulfate, heparin, heparin sulfate, heparan sulfate, gellan gum, xanthan gum, guar gum, water soluble cellulose derivatives, poly-glycolic acid, PLGA (poly-lactic-co-glycolic acid), collagen, polyhydroxyalkanoates (PHA), poly-ε-caprolactone, poly-ortho esters, poly-anhydrides, poly-phosphazenes, poly-amino acids, polydimethylsiloxane, polyurethranes, poly-tetrafluoroethylene, polyethylene, polysulphone, poly-methyl methacrylate, poly-2-hydroxyethylmethacrylate, polyamides, polypropylene, poly-vinyl chloride, polystyrene, poly-vinyl pyrrolidone and carrageenan.

### Claim 9 (depends on 1)

The method of claim 1, wherein the dendrimer is selected from the group consisting of a polyamidoamine (PAMAM) dendrimer, a polypropylenimine (PPI) dendrimer, a phosphorylated dendrimer, a polylysine dendrimer, a polyethylenimine dendrimer, an iptycene dendrimer, an aliphatic poly(ether) dendrimer, an aromatic polyether dendrimer, and a polypropylamine (POPAM) dendrimer.