Separation devices, associated methods, and systems

A method for clarifying a bioprocess fluid comprising particles suspended in a cell culture fluid, the method comprising:

• flowing an unclarified bioprocess fluid from a bioreactor through a plurality of mesofluidic channels of a separation device comprising a fluid inlet and fluid outlet to separate at least a portion of particles of the unclarified bioprocess fluid to generate a substantially clarified bioprocess fluid, the substantially clarified bioprocess fluid having more than 80% of the particles removed relative to the unclarified bioprocess fluid;
• and
• collecting the substantially clarified bioprocess fluid from the fluid outlet of the separation device;
• wherein a residence time of the bioprocess fluid between the fluid inlet and fluid outlet of the separation device ranges from 10 minutes to 40 minutes, and wherein the separation device is operated at an angle less than 10° relative to a work surface thereby allowing the particles to settle and collect on a lower interior surface of each mesofluidic channel.