# US 11,028,348 B2 · Naturally-derived antimicrobial cleaning solutions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,028,348 B2 - Document ID: US11028348B2 - Status: Granted - Kind: B2 - Application number: 15/938,676 - Filed: 2018-03-28 - Published: 2018-08-02 - Granted: 2021-06-08 - Earliest claimed: 2014-10-09 - Assignees: Pronatural Brands, LLC - Applicants: ProNatural Brands LLC - Inventors: William Salminen; Gary M. Russotti; Richard Aab; Robert Tuchrelo; Jeffrey Cahoon - Web page: https://patentgrep.com/patent/US11028348B2 ## CPC - C11D 3/48: Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions - A01N 25/30: characterised by the surfactants - A01N 31/02: Acyclic compounds - A01N 37/36: containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids - A01N 59/02: Sulfur; Selenium; Tellurium; Compounds thereof - C11D 1/146: {Sulfuric acid esters} - C11D 3/0031: {Carpet, upholstery, fur or leather cleansers} - C11D 3/201: {linear} - C11D 3/2075: {Carboxylic acids-salts thereof} - C11D 3/2079: {Monocarboxylic acids-salts thereof} - C11D 3/2082: {Polycarboxylic acids-salts thereof} - C11D 3/2086: {Hydroxy carboxylic acids-salts thereof} - C11D 2111/12: Soft surfaces, e.g. textile - C11D 2111/14: Hard surfaces - Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Various aspects of the embodiments relate to methods of cleaning and disinfecting with a mixture comprising a cleaning and disinfecting composition concentrate and water. The cleaning and disinfecting composition concentrate typically comprises an organic acid, a surfactant, and an alcohol, and the concentrate typically lacks any halogenated molecule. ## Claims ### Claim 1 A method of chemically ablating biofilm, comprising contacting the biofilm with a mixture comprising a cleaning and disinfecting composition concentrate and water, wherein: the cleaning and disinfecting composition concentrate comprises an organic acid, a fatty acid, a surfactant, an alcohol, and water; the organic acid is selected from one or more of citric acid, acetic acid, ascorbic acid, fumaric acid, propionic acid, oxalic acid, lactic acid, malic acid, and benzoic acid; the cleaning and disinfecting composition concentrate comprises the organic acid at a concentration of 15% to about 35% (w/v); the fatty acid is selected from one or more saturated or unsaturated C6-C18 monocarboxylic acids; the cleaning and disinfecting composition concentrate comprises the fatty acid at a concentration of about 1% to about 3% (w/v); the surfactant is a lauryl sulfate; the cleaning and disinfecting composition concentrate comprises the surfactant at a concentration of about 3% to about 35% (w/v); the alcohol is selected from ethanol, isopropanol, and n-propanol; the cleaning and disinfecting composition concentrate comprises the alcohol at a concentration of about 2% to about 10% (w/v); the cleaning and disinfecting composition concentrate is essentially free of halogenated molecules; the cleaning and disinfecting composition concentrate is essentially free of oxidizing agents other than hydrogen peroxide; the pH of the cleaning and disinfecting composition concentrate is less than 6.0; the mixture comprises the cleaning and disinfecting composition concentrate and water at a ratio of about 1:10 to about 1:128; and the biofilm is a biofilm of at least one organism selected from the group consisting of Pseudomonas aeruginosa, Escherichia coli, Listeria monocytogenes, Streptococcus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Salmonella enterica, Dekkera bruxellensis, Vancomycin Resistant Enterococcus faecalis (VRE), Clostridium difficile, Legionella pneumophilia, Stenotrophomonas maltophilia, Carbapenem-Resistant Enterobacter cloacae (CRE), Enterobacter aerogenes, Neisseria gonorrhoeae, and Candida albicans. ### Claim 2 (depends on 1) The method of claim 1, wherein the cleaning and disinfecting composition concentrate comprises: the organic acid at a concentration of about 28% to about 32% (w/v); the surfactant at a concentration of about 10% to about 15% (w/v); and/or the alcohol at a concentration of about 5% to about 6% (w/v). ### Claim 3 (depends on 1) The method of claim 1, wherein the mixture comprises the cleaning and disinfecting composition concentrate and water at a ratio of about 1:32 to about 1:128. ### Claim 4 (depends on 1) The method of claim 1, wherein the biofilm is a Pseudomonas aeruginosa biofilm. ### Claim 5 (depends on 1) The method of claim 1, wherein the biofilm is an Escherichia coli, Listeria monocytogenes, or Streptococcus biofilm. ### Claim 6 (depends on 1) The method of claim 1, further comprising mixing the cleaning and disinfecting composition concentrate and water, thereby resulting in the mixture. ### Claim 7 (depends on 1) The method of claim 1, wherein the mixture displays at least 99.9% efficacy relative to a control mixture at killing E. coli, S. aureus, Listeria, and Salmonella bacterial biofilms as assessed by one or more American Society for Testing and Materials (ASTM) protocols selected from ASTM E1053, ASTM E1153, ASTM E2315, and ASTM E2871. ### Claim 8 (depends on 1) The method of claim 1, wherein: the biofilm is a Staphylococcus aureus biofilm; and the mixture reduces Staphylococcus aureus cell count by at least 99.9%, relative to a time zero control, upon contacting the Staphylococcus aureus with the mixture for 2 minutes or more, thereby displaying efficacy at killing gram-positive bacteria. ### Claim 9 (depends on 1) The method of claim 1, wherein: the biofilm is a Staphylococcus aureus and an Escherichia coli biofilm; and the mixture reduces Staphylococcus aureus cell count by at least 99.9%, relative to a time zero control, upon contacting the Staphylococcus aureus with the mixture for 30 seconds or more, thereby displaying efficacy at killing gram-positive bacteria; and the mixture reduces Escherichia coli cell count by at least 99.9%, relative to a time zero control, upon contacting the Escherichia coli with the mixture for 30 seconds or more, thereby displaying efficacy at killing gram-negative bacteria. ### Claim 10 (depends on 1) A method of killing pathogens or microbes in a biofilm, comprising performing the method of claim 1, wherein: the pathogens or microbes are selected from Pseudomonas aeruginosa, Vancomycin Resistant Enterococcus faecalis (VRE), Clostridium difficile, Legionella pneumophilia, Acinetobacter baumannii, Stenotrophomonas maltophilia, Carbapenem-Resistant Enterobacter cloacae (CRE), Enterobacter aerogenes, Neisseria gonorrhoeae, and Candida albicans; and the method is effective at killing at least 99% of the pathogens or microbes in a biofilm. ### Claim 11 (depends on 1) The method of claim 1, wherein: the biofilm is a Escherichia Coli biofilm; and the mixture reduces Escherichia Coli cell count by at least 99.9%, relative to a time zero control, upon contacting the Escherichia Coli with the mixture for 2 minutes or more, thereby displaying efficacy at killing gram-negative bacteria. ### Claim 12 (depends on 1) The method of claim 1, wherein: the biofilm is a Klebsiella pneumoniae biofilm; and the mixture reduces Klebsiella pneumoniae cell count by at least 99.9%, relative to a time zero control, upon contacting the Klebsiella pneumoniae with the mixture for 10 minutes or more, thereby displaying efficacy at killing Klebsiella pneumonia. ### Claim 13 (depends on 1) The method of claim 1, wherein: the biofilm is a Acinetobacter baumannii biofilm; and the mixture reduces Acinetobacter baumannii cell count by at least 99.9%, relative to a time zero control, upon contacting the Acinetobacter baumannii with the mixture for 10 minutes or more, thereby displaying efficacy at killing Acinetobacter baumannii. ### Claim 14 (depends on 1) The method of claim 1, wherein: the biofilm is a Listeria monocytogenes biofilm; and the mixture reduces Listeria monocytogenes cell count by at least 99.9%, relative to a time zero control, upon contacting the Listeria monocytogenes with the mixture for 2 minutes or more, thereby displaying efficacy at killing Listeria. ### Claim 15 (depends on 1) The method of claim 1, wherein: the biofilm is a Salmonella enterica biofilm; and the mixture reduces Salmonella enterica cell count by at least 99.9%, relative to a time zero control, upon contacting the Salmonella enterica with the mixture for 5 minutes or more, thereby displaying efficacy at killing Salmonella. ### Claim 16 (depends on 1) The method of claim 1, wherein: the biofilm is a Dekkera bruxellensis biofilm; and the mixture reduces Dekkera bruxellensis cell count by at least 99.9%, relative to a time zero control, upon contacting the Dekkera bruxellensis with the mixture for 5 minutes or more, thereby displaying efficacy at killing fungi. ### Claim 17 (depends on 1) The method of claim 1, wherein: the biofilm is a Pseudomonas aeruginosa biofilm; and the mixture reduces Pseudomonas aeruginosa cell count by at least 99.75%, relative to a time zero control, upon contacting the Pseudomonas aeruginosa with the mixture for 10 minutes or more, thereby displaying efficacy against biofilms.