# US 11,028,152 B2 · Single domain antibodies to Chikungunya virus

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- Number: US 11,028,152 B2
- Document ID: US11028152B2
- Status: Granted
- Kind: B2
- Application number: 16/513,881
- Filed: 2019-07-17
- Published: 2020-01-23
- Granted: 2021-06-08
- Earliest claimed: 2018-07-20
- Assignees: The Government of the United States of America, as represented by the Secretary of the Navy
- Applicants: The Government of the United States of America, as represented by the Secretary of the Navy
- Inventors: Jinny Lin Liu; George P. Anderson; Ellen R. Goldman
- Web page: https://patentgrep.com/patent/US11028152B2

## CPC

- C07K 16/116: Togaviridae (F); Matonaviridae (F); Flaviviridae (F)
- A61P 31/14: for RNA viruses
- G01N 33/56983: {Viruses}
- C07K 2317/22: from camelids, e.g. camel, llama or dromedary
- C07K 2317/569: Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
- C07K 2317/76: Antagonist effect on antigen, e.g. neutralization or inhibition of binding
- C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
- C12N 2770/36111: Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
- C12N 2770/36123: Virus like particles [VLP]
- G01N 2333/181: Alphaviruses or Group A arboviruses, e.g. sindbis, VEE, EEE, WEE or semliki forest virus
- G01N 2469/10: Detection of antigens from microorganism in sample from host
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Described herein are single-domain antibodies that might serve as alternatives to conventional monoclonal antibodies for either the detection or treatment of Chikungunya Virus (CHIKV).

## Claims

### Claim 1

An isolated antibody comprising a protein sequence selected from the group consisting of SEQ ID Nos. 1-14.

### Claim 2 (depends on 1)

The isolated antibody of claim 1, wherein the protein sequence is selected from the group consisting of SEQ ID No. 1 and SEQ ID No. 13.

### Claim 3 (depends on 1)

The isolated antibody of claim 1, wherein the protein sequence is SEQ ID No: 1.

### Claim 4

A method of detecting Chikungunya virus, comprising:
contacting a sample known or suspected to contain Chikungunya virus with a bound or immobilized antibody that includes a protein sequence selected from the group consisting of SEQ ID Nos. 1-14 under conditions that permit antigen binding thereto; and
rising the antibody to remove unbound reagents, wherein
at least a portion of any Chikungunya virus in the sample remains bound to the antibody, thereby producing a response indicative of the presence of Chikungunya virus in the sample.

### Claim 5 (depends on 4)

The method of claim 4, wherein the protein sequence is selected from the group consisting of SEQ ID No. 1 and SEQ ID No. 13.

### Claim 6 (depends on 4)

The method of claim 4, wherein the protein sequence is SEQ ID No: 1.