# US 11,028,148 B2 · Recombinant collagen and elastin molecules and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,028,148 B2 - Document ID: US11028148B2 - Status: Granted - Kind: B2 - Application number: 16/839,042 - Filed: 2020-04-02 - Published: 2020-08-06 - Granted: 2021-06-08 - Earliest claimed: 2017-09-28 - Assignees: Geltor, Inc. - Applicants: Geltor, Inc. - Inventors: Nikolay Ouzounov; Alexander Lorestani; Monica Bhatia - Web page: https://patentgrep.com/patent/US11028148B2 ## CPC - C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] - A61K 8/65: Collagen; Gelatin; Keratin; Derivatives or degradation products thereof - A61K 38/39: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG] - A61Q 17/04: Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations - A61Q 19/08: Anti-ageing preparations - C07K 2319/20: containing a tag with affinity for a non-protein ligand - C07K 2319/60: containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP] - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS ## Abstract This disclosure provides non-naturally occurring collagen and elastin molecules. The non-naturally occurring collagens and elastins include truncated collagens, truncated elastins, as well as fusion proteins thereof. The non-naturally occurring collagen and elastin are useful in foods, cosmetics and many other products and uses. ## Claims ### Claim 1 A recombinant polypeptide comprising the amino acid sequence of SEQ ID NO: 1 having an internal truncation of from 50 amino acids to 250 amino acids. ### Claim 2 (depends on 1) The recombinant polypeptide of claim 1, wherein the recombinant polypeptide comprises the amino acid sequence according to SEQ ID NO: 91 or a fragment thereof. ### Claim 3 (depends on 1) The recombinant polypeptide of claim 1, wherein the recombinant polypeptide consists of the amino acid sequence according to SEQ ID NO: 91. ### Claim 4 (depends on 1) The recombinant polypeptide of claim 1, further comprising one or more selected from the group consisting of: a secretion tag, a histidine tag, a fluorescent protein, a protease cleavage site, and a beta-lactamase protein. ### Claim 5 (depends on 4) The recombinant polypeptide of claim 4, wherein the secretion tag is DsbA. ### Claim 6 (depends on 1) The recombinant polypeptide of claim 1, further comprising one or more GEK amino acid trimer repeats, one or more GDK trimer repeats, or a combination thereof. ### Claim 7 (depends on 1) A composition comprising from 0.005% w/w to 30% w/w of a recombinant polypeptide according to claim 1. ### Claim 8 (depends on 7) The composition of claim 7, wherein the recombinant polypeptide comprises the amino acid sequence according to SEQ ID NO: 91 or a fragment thereof. ### Claim 9 (depends on 7) The composition of claim 7, wherein the recombinant polypeptide consists of the amino acid sequence according to SEQ ID NO: 91. ### Claim 10 (depends on 7) The composition of claim 7, wherein the composition is formulated for topical application. ### Claim 11 (depends on 10) The composition of claim 10, wherein the composition comprises a topical carrier, a preservative, or both. ### Claim 12 (depends on 11) The composition of claim 11, wherein the topical carrier is selected from the group consisting of: a liposome, a biodegradable microcapsule, a lotion, a spray, an aerosol, a dusting powder, a biodegradable polymer, a mineral oil, a triglyceride oil, a silicone oil, glycerin, glycerin monostearate, an alcohol, an emulsifying agents, a liquid petroleum, a white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene, wax, sorbitan monostearate, polysorbate, cetyl ester wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol, cyclomethicone, cyclopentasiloxane, water, and any combination thereof. ### Claim 13 (depends on 11) The composition of claim 11, wherein the preservative is selected from the group consisting of: tocopherol, diiodomethyl-p-tolylsulfone, 2-bromo-2-nitropropane-1,3-diol, cis isomer 1-(3-chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, glutaraldehyde, 4,4-dimethyl oxazolidine, 7-ethylbicyclooxazolidine, methyl paraben, sorbic acid, rosemary extract, ethylenediaminetetraacetic acid (EDTA), and any combination thereof. ### Claim 14 (depends on 10) The composition of claim 10, wherein the composition is a cosmetic. ### Claim 15 (depends on 10) A method of treating the skin of a subject, the method comprising administering to the skin of a subject a composition according to claim 10, thereby treating the skin of the subject. ### Claim 16 (depends on 15) The method of claim 15, wherein the treating results in an increase in viability of keratinocytes, fibroblasts, or both, of the subject after exposure to ultraviolet (UV) radiation. ### Claim 17 (depends on 15) The method of claim 15, wherein the treating results in an increase in viability of keratinocytes, fibroblasts, or both, of the subject after exposure to urban dust. ### Claim 18 (depends on 15) The method of claim 15, wherein the treating results in a decrease in production of inflammatory cytokines. ### Claim 19 (depends on 15) The method of claim 15, wherein the subject is a human.