Methods for extracting proteins from a blood-based material

A method of producing an immunoglobulin G product from a blood-based material, the method comprising:

• applying the blood-based material to a first fractionation module, wherein a first salt is added to the blood-based material to produce a first intermediate, wherein the salt comprises between 11-20 wt % of the first intermediate;
• and wherein the first intermediate is separated to produce a first supernatant and a first paste;
• transferring the first supernatant to a second fractionation module, wherein a second salt is added to the first supernatant to produce a second intermediate, wherein the second salt comprises between 15-30 wt % of the second intermediate;
• and wherein the second intermediate is separated to produce a second supernatant and a second paste;
• dissolving the second paste to generate a dissolved second paste;
• transferring the dissolved second paste to a viral inactivation module and applying a virus inactivating agent to the dissolved second paste to generate a treated dissolved second paste;
• and
• transferring the treated dissolved second paste to a first chromatography module wherein the treated dissolved second paste is separated by a first chromatography process comprising an anion exchange resin to produce a first flow-through and a first eluate,
• wherein the first flowthrough comprises immunoglobulin G.