# US 11,027,274 B2 · Microfluidic cartridge and stacked testing assembly with microfluidic cartridge thereof

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- Number: US 11,027,274 B2
- Document ID: US11027274B2
- Status: Granted
- Kind: B2
- Application number: 15/880,504
- Filed: 2018-01-25
- Published: 2018-08-02
- Granted: 2021-06-08
- Earliest claimed: 2017-01-25
- Assignees: Foxconn Interconnect Technology Limited; Prenosis, Inc.
- Applicants: Foxconn Interconnect Technology Limited; Prenosis, Inc.
- Inventors: Bobby Reddy, Jr.; Rashid Bashir; Samuel Wachspress; Lauren Penrose; Chun-Yi Chang; Been-Yang Liaw
- Web page: https://patentgrep.com/patent/US11027274B2

## CPC

- B01L 3/502707: {characterised by the manufacture of the container or its components}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- G01N 33/5438: {Electrodes}
- B01L 2200/025: Align devices or objects to ensure defined positions relative to each other
- B01L 2200/027: for microfluidic devices
- B01L 2200/028: Modular arrangements
- B01L 2200/0652: Sorting or classification of particles or molecules
- B01L 2300/0645: Electrodes
- B01L 2300/0681: Filter
- B01L 2300/0883: Serpentine channels
- B01L 2300/0887: Laminated structure
- B01L 2400/086: using baffles or other fixed flow obstructions
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A stacked testing assembly (100) includes a microfluidic cartridge (10) for analyzing a fluid sample and a testing setup, said microfluidic cartridge includes a number of layers (1, 2) stacked in a height direction with many different kinds of combinations, said testing setup (20) is capable of assembling and testing all kinds of said layers combinations with no change to the setup.

## Claims

### Claim 1

A microfluidic cartridge for analyzing fluid samples, comprising:
a plurality of layers stacked in a height direction; wherein
each of said layers defines a standard number of via ports passing therethrough in the height direction for connecting non-adjacent layers to one another and being closed on the respective layers only if the respective layers need to receive fluid sample from said non-adjacent layers, and a standard number of alignment holes passing therethrough in the height direction and locating at corners thereof to align the layers during assembly of said layers; wherein
at least one of said layers defines a standard number of input ports recessing from one side thereof in the height direction while not penetrating the other opposite side thereof, a standard number of output ports passing therethrough in the height direction and corresponding to the input ports, and flow channels connecting between the respective input ports and output ports; said fluid samples enter the layer via the input ports, flowing in the layer via the flow channels and exit of the layer via the output ports; wherein
said layers includes a first layer at least includes said via ports and alignment holes, and a second layer includes said via ports, alignment holes, input ports, output ports and flow channels; said first layer stacks on an upper side of the second layer; said first layer is an electrode layer and defines a set of electrodes arranged on a lower side thereof; said second layer is a counter layer and defines flow channels on an upper side thereof, each flow channel forms a detecting region for said fluid sample passing singly, the electrodes stride across the detecting region to analyze the fluid sample; wherein
the flow channels include a pair of first flow channels and a pair of second flow channels; wherein
said plurality of layers further include another layer stacked on an upper side of the first layer and defining a capture chambers for capturing the selective particles from the fluid sample of interest; wherein
the first layer, the second layer and said another layer are arranged with one another to allow the fluid sample of interest to sequentially pass through the pair of first flow channels in the second layer, the capture chambers in said another layer, and the pair of second flow channels in the second layer.