Systems and methods for utilizing crossflow filtration for cell enrichment

A disposable cell enrichment kit, comprising:

• a crossflow filtration device configured to be disposed along a main loop pathway and configured to receive a process volume containing a biological sample and to utilize crossflow filtration, via a micro-porous membrane, to retain a specific cell population in a retentate from the process volume and to remove a permeate including specific biological components from the process volume, wherein the crossflow filtration device comprises a laminated filtration unit comprising:
• the micro-porous membrane;
• a first mating portion, the first mating portion and the micro-porous membrane together defining at least one channel configured to receive the process volume containing the biological sample, the at least one channel extending parallel to the micro-porous membrane;
• a second mating portion;
• a membrane support comprising a first plurality of structural features that define a first plurality of openings, wherein the first plurality of structural features are coupled to and provide support to the micro-porous membrane, and the first plurality of openings allow the permeate to flow through them after crossing the micro-porous membrane;
• and
• at least one inlet in the first mating portion, the at least one inlet being in fluid communication with the at least one channel and being configured to direct the process volume into the at least one channel in a direction generally parallel to the micro-porous membrane;
• wherein each inlet of the at least one inlet in the first mating portion is positioned adjacent to a first end of the channel so as to direct the process volume into the at least one channel at a point adjacent to the first end of the channel;
• and
• wherein each inlet of the at least one inlet extends in a continuous manner substantially across a width of the channel so as to provide a substantially uniform velocity across the width of the single channel;
• and
• wherein the membrane support is configured to be disposed between the micro-porous membrane and either the first mating portion or the second mating portion.