# US 11,027,044 B2 · Method for producing sheet-like cell structure and sheet-like cell structure > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,027,044 B2 - Document ID: US11027044B2 - Status: Granted - Kind: B2 - Application number: 15/941,187 - Filed: 2018-03-30 - Published: 2018-10-04 - Granted: 2021-06-08 - Earliest claimed: 2015-09-30 - Assignees: FUJIFILM Corporation - Applicants: FUJIFILM Corporation - Inventors: Kentaro Nakamura - Web page: https://patentgrep.com/patent/US11027044B2 ## CPC - A61L 27/38: {containing added animal cells (organs or tissue containing native cells A61L27/36)} - A61L 27/222: {Gelatin} - A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} - A61L 27/52: Hydrogels or hydrocolloids - C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] - C12N 2533/00: Supports or coatings for cell culture, characterised by material - C12N 2533/30: Synthetic polymers - C12N 2533/40: Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers - C12N 2533/54: Collagen; Gelatin - C12N 2533/72: Chitin, chitosan - C12N 2533/78: Cellulose - C12N 2533/80: Hyaluronan - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract An object of the present invention is to provide a method for producing a sheet-like cell structure having excellent strength and shape-maintaining performance, and a sheet-like cell structure having excellent strength and shape-maintaining performance. According to the present invention, there is provided a method for producing a sheet-like cell structure, including: a step of adding a biocompatible macromolecular block, a cell, and a liquid medium onto a culture support body having a plurality of recessed portions on a culture surface, and immersing the biocompatible macromolecular block and the cell in uppermost portions of the recessed portions; and a step of culturing the cell to obtain a sheet-like cell structure. ## Claims ### Claim 1 A method for producing a sheet-like cell structure, comprising: adding a biocompatible macromolecular block, a cell, and a liquid medium onto a culture support body having a plurality of recessed portions on a culture surface, and immersing the biocompatible macromolecular block and the cell in uppermost portions of the recessed portions; and culturing the cell to obtain a sheet-like cell structure which maintains its shape and resists rolling; wherein the culture surface of the culture support body is subjected to a treatment for suppressing adhesion of cells, the area of the recessed portions on the culture surface is larger than or equal to 70% with respect to the whole area of the culture surface, and the recessed portions have a depth of from 10 μm to 2,000 μm. ### Claim 2 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein the recessed portions have a diameter of from 10 μm to 2,000 μm, and a circular shape. ### Claim 3 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein a thickness of a thinnest portion of the sheet-like cell structure is 50 μm to 5 mm. ### Claim 4 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein a size of the biocompatible macromolecular block is 1 μm to 700 nm. ### Claim 5 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein biocompatible macromolecules are recombinant gelatin. ### Claim 6 (depends on 5) The method for producing a sheet-like cell structure according to claim 5, wherein the recombinant gelatin is any one of a peptide formed of an amino acid sequence described in SEQ ID NO: 1; a peptide which is formed of an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence described in SEQ ID NO: 1, and has biocompatibility; and a peptide which is formed of an amino acid sequence having 90% or more sequence identity to the amino acid sequence described in SEQ ID NO: 1, and has biocompatibility. ### Claim 7 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein the cells are cultured at 35° C. to 40° C. ### Claim 8 (depends on 1) The method for producing a sheet-like cell structure according to claim 1, wherein the recessed portions are circular, and have a depth of 100 μm to 2,000 μm and a diameter of 200 μm to 2,000 μm. ### Claim 9 A sheet-like cell structure comprising: a biocompatible macromolecular block; and a cell, wherein the sheet-like cell structure has a plurality of protruding portions on at least a single surface thereof, and a plurality of the biocompatible macromolecular blocks are arranged in gaps between a plurality of the cells in the protruding portions; the sheet-like cell structure maintains its shape and resists rolling; the protruding portions have a height of from 10 μm to 2,000 μm; and the protruding portions have an area that is larger than or equal to 70% with respect to the area of said single surface of the sheet-like cell structure. ### Claim 10 (depends on 9) The sheet-like cell structure according to claim 9, further comprising: protruding portions having a height of 10 μm to 2,000 μm and a diameter of 10 μm to 2,000 μm. ### Claim 11 (depends on 9) The sheet-like cell structure according to claim 9, wherein the protruding portions have a height of 100 μm to 2,000 μm and a diameter of 200 μm to 2,000 μm.