# US 11,026,981 B2 · LDL-cholesterol-lowering cell extract and food supplement > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,026,981 B2 - Document ID: US11026981B2 - Status: Granted - Kind: B2 - Application number: 16/621,744 - Filed: 2018-06-14 - Published: 2020-04-02 - Granted: 2021-06-08 - Earliest claimed: 2017-06-15 - Assignees: ezCol B.V. - Applicants: ezCol B.V. - Inventors: Ruddy Wattiez; Felice Mastroleo; Robertus Christiaan Josephus Suters - Web page: https://patentgrep.com/patent/US11026981B2 ## CPC - A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00) - A23L 33/13: Nucleic acids or derivatives thereof (A23L33/145 takes precedence) - A61K 9/0056: {Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals} - A61K 47/06: Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite - A61P 1/16: for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics - A61P 3/06: Antihyperlipidemics - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract The present invention relates to a low-density lipoprotein (LDL)-cholesterol-lowering preparation, in particular to a bacterial cell extract for use as a medicament for lowering of the blood plasma LDL-cholesterol level, wherein the extract is obtained by extraction for between 10 minutes and 48 hours at between 8° C. and 37° C., while mixing the cells with the mixture, and wherein the ldl-cholesterol-lowering cell extract and food supplementldl-cholesterol-lowering cell extract and food supplement Rhodospirillum rubrum cells are extracted with the mixture in a volume ratio of between 10:1 and 1:10 of petroleum ether with a boiling point of between 60° C. and 80° C. and methanol comprising sodium chloride. More specifically, the present invention relates to a petroleum ether extract of Rhodospirillum rubrum obtainable by extraction of Rhodospirillum rubrum cells with a mixture of petroleum ether with a boiling point of between 60° C. and 80° C. and methanol comprising sodium chloride. The invention also relates to the petroleum ether extract of Rhodospirillum rubrum of the invention, for use in a method for the lowering of LDL-cholesterol in blood plasma of a subject. The invention further relates to a pharmaceutical preparation, a food supplement containing said preparation, a foodstuff containing said food supplement, and to a method for their preparation. ## Claims ### Claim 1 An extract of Rhodospirillum rubrum that is obtained by extracting R. rubrum with a solvent mixture of petroleum ether and methanol containing sodium chloride, wherein the petroleum ether has boiling point between 60° C. and 80° C. wherein the R. rubrum is extracted for a period of time between 10 minutes and 48 hours at a temperature between 8° C. and 37° C., while mixing the R. rubrum cells with the solvent mixture, and wherein the ratio of petroleum ether to methanol comprising sodium chloride in a solvent mixture is between 10:1 and 1:10. ### Claim 2 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the extract is obtained by extraction for between 20 minutes and 24 hours, at between 10° C. and 30° C. ### Claim 3 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the extract is obtained upon centrifugation for between about ten minutes and 60 minutes, at between 15° C. and 25° C., after incubation of the Rhodospirillum rubrum cells in the solvent mixture. ### Claim 4 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the ratio of petroleum ether to methanol comprising sodium chloride in the solvent mixture is between 8:1 and 1:8. ### Claim 5 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the extract is obtained by extraction for between 1 hour and 3 hours at a temperature of between 15° C. and 25° C., and wherein the ratio of petroleum ether to methanol containing sodium chloride in the solvent mixture is 1:1. ### Claim 6 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the amount of sodium chloride in the methanol in the solvent mixture is between 0.02% and 0.04% by weight. ### Claim 7 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein said extract comprises one or more carotenoids and/or one or more quinones. ### Claim 8 (depends on 7) The petroleum ether extract of Rhodospirillum rubrum according to claim 7, wherein said extract comprises one or more carotenoids selected from the group consisting of rhodovibrin, 1-hydroxy-spirilloxanthin, 3,4-didehydrorhodopin, chloroxanthin, bacteriopheophytin a, rhodopin, spirilloxanthin, 3,4-dihydrospirilloxanthin and phytyl derivative of bacteriopheophytin a, and/or one or more quinones selected from the group consisting of ubiquinol-10, ubiquinone-9, ubiquinone-10 and rhodoquinone-10. ### Claim 9 (depends on 8) The petroleum ether extract of Rhodospirillum rubrum according to claim 8, wherein said extract comprises rhodovibrin, 1-hydroxy-spirilloxanthin, 3,4-didehydrorhodopin, chloroxanthin, bacteriopheophytin a, rhodopin, spirilloxanthin, 3,4-dihydrospirilloxanthin, phytyl derivative of bacteriopheophytin a, ubiquinol-10, ubiquinone-9, ubiquinone-10 and rhodoquinone-10. ### Claim 10 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum ccording to claim 1, for use as a medicament. ### Claim 11 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, for use in the lowering of LDL-cholesterol in blood plasma of a subject. ### Claim 12 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, for use in a method for the lowering of LDL-cholesterol in blood plasma of a subject. ### Claim 13 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, for use in the lowering of LDL-cholesterol in blood plasma of a subject or for use in a method for the lowering of LDL-cholesterol in blood plasma of a subject, wherein the subject surfers from or has an increased risk for any one or more of cardiovascular disease, atherosclerosis, dyslipidemia, arteriosclerosis, hypercholesterolemia, familial hypercholesterolemia, hyperlipidemia, an LDL plasma level of at least 70 mg/dL, a total cholesterol level of at least 200 mg/dL, an Lp(a) level of at least 14 mg/dL, inflammation, inflammatory disease, ischemia, or infection. ### Claim 14 (depends on 1) The petroleum ether extract of Rhodospirillum rubrum according to claim 1, wherein the extract is administered orally. ### Claim 15 (depends on 1) A food supplement with LDL-cholesterol lowering properties, comprising the petroleum ether extract of Rhodospirillum rubrum according to claim 1. ### Claim 16 (depends on 15) A foodstuff comprising the food supplement according to claim 15. ### Claim 17 (depends on 1) A method for the production of the petroleum ether extract of Rhodospirillum rubrum of claim 1, comprising the steps of: a) providing Rhodospirillum rubrum cells b) providing a biphasic mixture having about a 1:1 ratio by volume of petroleum ether that has a boiling point between 60° C. and 80° C. and methanol that comprises about 0.03% by weight of sodium chloride; c) mixing the cells of step a) with the biphasic mixture of step b) for about 1 to 3 hours at a temperature between 15° C. and 25° C.; d) separating the petroleum ether phase from the methanol phase of the biphasic mixture; and e) isolating the petroleum ether phase thereby, obtaining the petroleum ether extract of Rhodospirillum rubrum.