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US 11,026,610 B2Granted
Method of producing thin enzyme-based sensing layers on planar sensors
AssigneeCalifornia Institute of Technology
InventorsSamson Chen, Axel Scherer, Dvin Adalian, Peter Petillo, Muhammad Musab Jilani, Xiomara L. Madero, Deepan Kishore Kumar
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Abstract
A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.
Claims14
A method, comprising:
- depositing a masking layer onto a device comprising a plurality of electrodes, wherein the masking layer covers the plurality of electrodes;
- depositing an adhesion promoter onto the device and onto the masking layer, wherein deposition of the adhesion promoter onto the plurality of electrodes is avoided due to the masking layer;
- removing the masking layer from the device coated with the adhesion promoter;
- after removing the masking layer, spin coating a signal transduction enzyme onto the device, the signal transduction enzyme being configured to detect an active compound capable of generating an electric current;
- and
- crosslinking the signal transduction enzyme by depositing a crosslinker onto the spin coated signal transduction enzyme to produce a cross-linked enzyme film having a thickness of less than 1 micrometer, and
- wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within 1 second.
4 more elements
Dependencies are inferred from the claim text.