Optical phase retrieval systems using color-multiplexed illumination

A method for recovering phase and amplitude data from an image of a sample, comprising:

• encoding a source of light into a partially coherent illumination that embeds multiple illumination patterns into a plurality of color channels each at distinct illumination wavelengths;
• directing said partially coherent illumination on the sample and modulating the sample by each illumination wavelength separately and independently of each other;
• for sensing all color channels simultaneously;
• generating a color image of the sample containing information about both phase and amplitude of the sample;
• and
• extracting quantitative amplitude and phase data from the color image of the sample by processing the image with a single deconvolution:
• wherein Ĩ is a color intensity measurement, ĨO is a background signal, N is the total number of wavelengths, Ã is amplitude, {tilde over (ϕ)} is phase, {tilde over (H)}ϕ,m and {tilde over (H)}A,m are transfer functions for phase and amplitude, respectively, for a given wavelength index m, and R(ϕ, A) is a regularizer function.

An apparatus for recovering phase and amplitude data from an image of a sample, comprising:

• (a) an encoded light source configured for providing a partially coherent illumination that embeds multiple illumination patterns into a plurality of color channels each at distinct illumination wavelengths;
• (b) one or more optical elements configured for directing said partially coherent illumination on the sample, wherein the sample is modulated by each illumination wavelength separately and independently of each other;
• (c) an optical imaging device configured for sensing all color channels simultaneously;
• (d) a processing unit;
• and
• (e) a non-transitory memory storing instructions executable by the processing unit;
• (f) wherein said instructions, when executed by the processing unit, perform steps comprising:
• (g) generating a color image of the sample containing information about both phase and amplitude of the sample;
• and
• (h) extracting quantitative amplitude and phase data from the color image of the sample by processing the image with a single deconvolution selected from the group consisting of (i) an L2 regularization and (ii) an L1 regularization on an object or object gradient.

A method for recovering phase and amplitude data from an image of a sample, comprising:

• encoding a source of light into a partially coherent illumination that embeds multiple illumination patterns into a plurality of color channels each at distinct illumination wavelengths;
• directing said partially coherent illumination on the sample and modulating the sample by each illumination wavelength separately and independently of each other;
• for sensing all color channels simultaneously;
• generating a color image of the sample containing information about both phase and amplitude of the sample;
• and
• extracting quantitative amplitude and phase data from the color image of the sample by processing the image with a single deconvolution selected from the group consisting of (i) an L2 regularization and (ii) an L1 regularization on an object or object gradient.