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US 11,021,727 B2Granted
Efficient production of steviol glycosides in recombinant hosts
AssigneeEvolva SA
InventorsErnesto Simon, Iben Nordmark Anderson, Michael Dalgaard Mikkelsen, Jorgen Hansen, Veronique Douchin
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Abstract
Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretions of steviol glucosides of interest.
Claims22
A recombinant microorganism capable of producing a steviol glycoside in a cell culture,
- wherein the microorganism has a modified expression of at least one endogenous transporter gene and at least one endogenous transcription factor gene that regulates expression of the at least one endogenous transporter gene,
- wherein the modified expression comprises an overexpression of at least one endogenous transporter gene encoding a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:115 and at least one endogenous transcription factor gene encoding a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:104, wherein the overexpresson is produced in the microorganism comprising replacing endogenous promoter with a constitutive or inducible promoter;
- wherein the microorganism further expresses at least one heterologous transporter gene;
- wherein the steviol glycoside is Rebaudioside A, Rebaudioside B, Rebaudioside D, Rebaudioside E, Rebaudioside M, or an isomer thereof.
1 more element
Dependencies are inferred from the claim text.