System and method for biosynthesis
AssigneeThe Regents of the University of California
InventorsTodd Prentice Coleman, Marianne Catanho, Phillip Kyriakakis
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Abstract
Methods, systems, and devices are disclosed for in vivo production or biosynthesis of metabolites in foreign cells using the combination of (i) one or more ferredoxin dependent enzyme(s) and (ii) a ferredoxin (Fd)/ferredoxin-NADP+ reductase (FNR) system. The ferredoxin dependent enzymes and the Fd/FNR system are from the same species or from a different but matching species.
Claims23
A system of in vivo production of a metabolite in a foreign host cell, comprising:
- one or more ferredoxin dependent enzymes targeting a specific subcellular location selected from cytoplasm, mitochondria and peroxisome;
- and
- a ferredoxin (Fd)/ferredoxin-NADP+ reductase (FNR) system targeting the same specific subcellular location, wherein:
- the metabolite and the one or more ferredoxin dependent enzymes are from the same species,
- the metabolite and the host cell are from different species, and
- the Fd/FNR system and the one or more ferredoxin dependent enzymes are from the same species.
A method of in vivo producing a metabolite in a foreign host cell comprising:
- providing to the host cell one or more ferredoxin dependent enzymes targeting a specific subcellular location selected from cytoplasm, mitochondria, and peroxisome, and a ferredoxin (Fd)/ferredoxin-NADP+ reductase (FNR) system (Fd-FNR system) targeting the same subcellular location;
- and
- culturing the host cell such that the metabolite is produced in the host cell, wherein:
- the metabolite and the one or more ferredoxin dependent enzymes are from the same species,
- the metabolite and the host cell are from different species, and
- the Fd/FNR system and the one or more ferredoxin dependent enzymes are from the same species.
A system of in vivo production of a metabolite in a foreign host cell comprising:
- one or more ferredoxin dependent enzymes targeting a specific subcellular location selected from cytoplasm, mitochondria, and peroxisome, and
- a ferredoxin (Fd)/ferredoxin-NADP+ reductase (FNR) system (Fd-FNR system) targeting the same specific subcellular location, wherein:
- the metabolite and the one or more ferredoxin dependent enzymes are from the same species,
- the metabolite and the host cell are from different species,
- the Fd/FNR system and the one or more ferredoxin dependent enzymes are from different species, and
- the amino acid sequence of a ferredoxin of the species of the one or more ferredoxin dependent enzymes and the amino acid sequence of a ferredoxin of the Fd/FNR species are at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or 100% identical.
A method of in vivo production of a metabolite in a foreign host cell comprising:
- transplanting into the host cell (a) one or more ferredoxin dependent enzyme(s) targeting a specific subcellular location selected from cytoplasm, mitochondria, and peroxisome, and (b) a ferredoxin (Fd)/ferredoxin-NADP+ reductase (FNR) system (Fd-FNR system) targeting the same specific subcellular location;
- and
- culturing the transplanted host cell such that the metabolite is produced in the host cell, wherein:
- the metabolite and the one or more ferredoxin dependent enzymes are from the same species,
- the metabolite and the host cell are from different species,
- the Fd/FNR system and the one or more ferredoxin dependent enzymes are from different species, and
- the amino acid sequence of the ferredoxin of the species of the one or more ferredoxin dependent enzymes and the amino acid sequence of the ferredoxin of the Fd/FNR species are at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or 100% identical.
Dependencies are inferred from the claim text.