Genetically engineered Pseudomonas strains capable of metabolizing ethylene glycol and its metabolic intermediates
AssigneesAlliance for Sustainable Energy, LLC, University of Stuttgart, UT-Battelle, LLC
InventorsGregg Tyler Beckham, Mary Ann Franden, Lahiru N. Jayakody, Nick Johannes Petrus Wierckx, Janosch Alexander Klebensberger, Lars Mathias Blank, Wing-Jin Li, Bernhard Hauer, Nicholas S. Cleveland, William E. Michener, Jason Michael Whitham
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Abstract
Presented herein are genetically engineered Pseudomonas strains capable of metabolizing ethylene glycol and producing polyhydroxyalkanoates.
Claims19
A genetically engineered Pseudomonas for growth on ethylene glycol as a sole carbon source comprising exogenous genes gcl (glyoxylate carboligase), hyi (hydroxypyruvate isomerase), glxR (tartronate semialdehyde reductase), PP_4300, pykF (pyruvate kinase), and glcDEF (glycolate oxidase operon).
A genetically engineered Pseudomonas that grows on ethylene glycol as a sole carbon source wherein the Pseudomonas comprises exogenous genes gcl, hyi, glxR, PP_4300, pykF, and glcDEF and that produces polyhydroxyalkanoates.
Dependencies are inferred from the claim text.