# US 11,020,497 B2 · Rod-shaped plant virus nanoparticles as imaging agent platforms

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- Number: US 11,020,497 B2
- Document ID: US11020497B2
- Status: Granted
- Kind: B2
- Application number: 16/149,828
- Filed: 2018-10-02
- Published: 2019-01-31
- Granted: 2021-06-01
- Earliest claimed: 2012-10-09
- Assignees: Case Western Reserve University
- Applicants: Case Western Reserve University
- Inventors: Nicole Steinmetz; Michael Bruckman
- Web page: https://patentgrep.com/patent/US11020497B2

## CPC

- A61K 49/1896: {not provided for elsewhere, e.g. cells, viruses, ghosts, red blood cells, virus capsides}
- A61K 49/1884: {Nanotubes, nanorods or nanowires}
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2770/00031: Uses of virus other than therapeutic or vaccine, e.g. disinfectant
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A rod-shaped plant virus having an interior surface and an exterior surface, and at least one imaging agent that is linked to the interior and/or exterior surface is described. The rod-shaped viruses can be combined into larger spherical nanoparticles. A rod-shaped plant virus or spherical nanoparticles including an imaging agent can be used in a method of generating an image of a tissue region of a subject such as a tumor or atherosclerotic tissue by administering the virus particle to the subject and generating an image of the tissue region of the subject to which the virus particle has been distributed.

## Claims

### Claim 1

A spherical nanoparticle imaging platform, comprising a spherical arrangement of the coat proteins of one or more rod-shaped plant virus particles linked to an imaging agent on an interior surface of the virus particle, formed by thermal transition of the rod-shaped virus particles, wherein the imaging platform has a longitudinal relaxivity of greater than about 10 mM−1S−1 per linked imaging agent when measured at 60 mHz at a physiological pH.

### Claim 2 (depends on 1)

The spherical nanoparticle imaging platform of claim 1, wherein the rod-shaped plant virus is a tobacco mosaic virus.

### Claim 3 (depends on 1)

The spherical nanoparticle imaging platform of claim 1, wherein the imaging agent is a chelated lanthanide.

### Claim 4 (depends on 1)

The spherical nanoparticle imaging platform of claim 1, wherein the imaging platform has a longitudinal relaxivity of greater than about 15 mM−1S−1 per linked imaging agent when measured at 60 mHz at a physiological pH.