Device and method for preparing and administering one-component fibrin sealant

A device for preparing and delivering fibrin sealant to a surface, the device comprising:

• a. a syringe comprising a barrel and a plunger, wherein the barrel contains a quantity of a cell-free liquid mixture comprising:
• fibrin and at least one small molecule inhibitor of fibrin clot formation;
• b. a resin bed comprising a size-exclusion chromatographic resin, the resin bed being disposed within a vessel of the device such that when an interior of the syringe barrel is in fluid communication with the resin bed, expulsion of the mixture from the barrel by the plunger forces the mixture to pass through the resin bed within the vessel resulting in reduction in a concentration of the inhibitor(s) within the mixture, wherein following the passage through the vessel, a fibrin clot is formed with a clotting time (TCLOT) of at most 1 hour at an ambient temperature selected from the group consisting of 21° C., 22° C., 23° C., 24° C., and 25° C.