Method for manufacturing useful substance in which high-density cultured strain of filamentous fungi is used
AssigneeTohoku University
InventorsKeietsu Abe, Katsuya Gomi, Kei Yoshimi
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Abstract
An object to be achieved by the present invention is to culture a filamentous fungus at a high density, thereby enabling mass production of a useful substance. The present invention provides a method of producing a substance, including the steps of: culturing a mutant filamentous fungus with no expression of α-1,3-glucan to allow the filamentous fungus to produce a substance; and collecting the resulting substance.
Claims9
A method for increasing the production amount of protein per unit volume in a mutant filamentous fungus which lacks α-1,3-glucan compared to the corresponding parent filamentous fungus comprising the steps of:
- culturing the mutant filamentous fungus to allow said fungus to undergo a hyphal growth and produce the protein;
- and collecting the produced protein,
- wherein the mutant filamentous fungus is a mutant Aspergillus oryzae, and wherein the mutant filamentous fungus is deficient in agsB gene encoding α-1,3-glucan synthase and further deficient in at least one α-1,3-glucan synthase ags gene other than the agsB gene, and wherein the culturing step is conducted in liquid culture medium.
A method for increasing the density of hyphae of a mutant filamentous fungus which lacks α-1,3-glucan compared to the corresponding parent filamentous fungus comprising the steps of:
- culturing the mutant filamentous fungus to allow for said fungus undergoing a hyphal growth to reach the increased level of the density of hyphae which is associated with protein production in said mutant filamentous fungus and
- collecting the produced protein,
- wherein the mutant filamentous fungus is a mutant Aspergillus oryzae, and wherein the mutant filamentous fungus is deficient in agsB gene encoding α-1,3-glucan and further deficient in at least one α-1,3-glucan synthase ags gene other than the agsB gene, and wherein the culturing step is conducted in liquid culture medium.
Dependencies are inferred from the claim text.