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US 11,013,798 B2Granted
Orf virus-based platform for vaccine delivery
AssigneesSouth Dakota Board of Regents, Federal University of Santa Maria
InventorsDiego G. Diel, Eduardo F. Flores
Life of this patent
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Abstract
The present invention is directed to novel vaccine delivery platform based on the Orf virus (ORFV) genome, which carry heterologous antigens, methods of making and methods of using the same for prevention of infections, diseases, and other conditions in animals.
Claims14
A vector for vaccine delivery in livestock comprising:
- a nucleic acid sequence encoding an infectious Orf virus operably linked to a heterologous nucleic acid sequence encoding at least one antigen, wherein the at least one antigen encoding sequence is inserted into an insertion site created by a deletion in ORFV024 and/or ORFV121 of a wild type Orf virus strain, and wherein the vector comprises:
- (a) SEQ ID NO:
- 4 or 5;
- (b) a polynucleotide that hybridizes with a sequence of (a) under stringent conditions defined as hybridizing to filter bound DNA on 0.5M NaHPO4, 7% SDS, 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS at 68° C.;
- (c) a polynucleotide that is at least 90% identical to the polynucleotide of (a);
- or
- (d) a polynucleotide that is at least 95% identical to the polynucleotide of (a).
4 more elements
A method of delivering a vaccine in livestock comprising:
- administering a nucleic acid construct comprising a nucleic acid sequence encoding an infectious Orf virus operably linked to a heterologous nucleic acid sequence encoding at least one antigen, wherein the at least one antigen is inserted into an insertion site created by a deletion in ORFV024 and/or ORFV121 of a wild type Orf virus, and wherein the nucleic acid construct comprises:
- (a) SEQ ID NO:
- 4 or 5;
- (b) a polynucleotide that hybridizes with a sequence of (a) under stringent conditions defined as hybridizing to filter bound DNA on 0.5M NaHPO4, 7% SDS, 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS at 68° C.;
- (c) a polynucleotide that is at least 90% identical to the polynucleotide of (a);
- or
- (d) a polynucleotide that is at least 95% identical to the polynucleotide of (a.
4 more elements
Dependencies are inferred from the claim text.