Method for preparing foot-and-mouth disease vaccines

A method for preparing a foot and mouth disease (FMD) vaccine, comprising the following steps:

• (i) obtaining cell culture media containing FMD virus;
• (ii) separating and purifying the cell culture media containing FMD virus by passing through an integrated filtration system with two membranes in combination, which comprises a microfiltration device and an ultrafiltration device arranged in parallel, wherein the microfiltration device comprises a microfiltration feed tank, a first main pump, and a microfiltration membrane, and the ultrafiltration device comprises a concentrate tank, a second main pump, and an ultrafiltration membrane;
• wherein the microfiltration device is configured to remove large-particle impurities in the cell culture media containing FMD virus, and the ultrafiltration device is configured to remove small molecular impurities in the cell culture media containing FMD virus;
• wherein the microfiltration device and the ultrafiltration device run simultaneously to perform microfiltration and ultrafiltration of the cell culture media containing FMD virus at the same time to thereby form a concentrate containing FMD virus in the concentrate tank;
• wherein the microfiltration device is connected to a first constant flow pump, and the ultrafiltration device is connected to a second constant flow pump so as to dynamically control the transmembrane flux, the transmembrane flux is 65-100% of the critical membrane flux of the microfiltration membrane or the ultrafiltration membrane, the volume of the concentrate tank is 1/5-1/50 of the initial volume of the cell culture media containing FMD virus, and the volume of the concentrate tank is equal to or not more than 1.5 times of the target concentrate volume of the cell culture media containing FMD virus;
• (iii) collecting the concentrate containing FMD virus obtained in step (ii).