Process for producing 1,3-propanediol compound
AssigneePhotanol B.V.
InventorsKlaas Jan Hellingwerf, Maarten Joost Teixeira De Mattos
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Abstract
A process of producing an organic compound and/or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting said culture to light, wherein said cell is capable of expressing a nucleic acid molecule, wherein the expression of said nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into said organic compound and/or into said intermediary compound and wherein said nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in said culture.
Claims12
A process of producing 1,3-propanediol compound, comprising:
- feeding carbon dioxide to a culture of Cyanobacterial cells;
- and
- subjecting the culture to light,
- wherein the cell expresses a nucleic acid molecule and expression of the nucleic acid molecule confers an ability to convert a glycolytic intermediate into 1,3-propanediol, wherein said nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in said culture, wherein the glycolytic intermediate is hydroxypropionaldehyde, wherein said nucleic acid molecule encodes an enzyme that converts glyceraldehyde-3-phosphate to 1,3-propanediol, wherein said enzyme is glycerol dehydratase, and wherein the glycerol dehydratase has an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 31 or is encoded by a polynucleotide sequence that is at least 95% identical to SEQ ID NO:
- 32.
A Cyanobacterial cell that expresses a nucleic acid molecule, wherein expression of the nucleic acid molecule allows the Cyanobacterial cell to convert a glycolytic intermediate into 1,3-propanediol, wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient when culturing said Cyanobacterial cell, wherein the glycolytic intermediate is hydroxypropionaldehyde, wherein said nucleic acid molecule encodes an enzyme that converts glyceraldehyde-3-phosphate to 1,3-propanediol, wherein said enzyme is a glycerol dehydratase, and wherein the glycerol dehydratase has an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 31 or is encoded by a polynucleotide sequence that is at least 95% identical to SEQ ID NO:
- 32.
Dependencies are inferred from the claim text.