Regulation of gene expression by temporal or leaf specific promoters
AssigneeMonsanto Technology LLC
InventorsEdwards M. Allen, Sara E. Heisel, Sergey Ivashuta, Elysia K. Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Abstract
This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
Claims13
A method for providing temporal or leaf specific expression of a gene in a plant, comprising:
- (i) operably linking to the gene a recombinant DNA construct encoding a promoter comprising:
- a. SEQ ID NO. 31,
- b. a sequence having at least 95% sequence identity to SEQ ID NO. 31,
- c. SEQ ID NO. 35,
- d. a sequence having at least 95% sequence identity to SEQ ID NO. 35,
- e. SEQ ID NO. 36, or
- f. a sequence having at least 95% sequence identity to SEQ ID NO. 36,
- wherein the promoter exhibits temporal or leaf specific activity;
- and
- (ii) transforming the recombinant DNA construct in the plant.
A recombinant DNA construct transcribable in a plant cell for providing temporal or leaf specific expression of a gene, comprising a gene operably linked to a promoter comprising:
- a. SEQ ID NO. 31,
- b. a sequence having at least 95% sequence identity to SEQ ID NO. 31,
- c. SEQ ID NO. 35,
- d. a sequence having at least 95% sequence identity to SEQ ID NO. 35,
- e. SEQ ID NO. 36, or
- f. a sequence having at least 95% sequence identity to SEQ ID NO. 36,
- wherein the promoter exhibits temporal or leaf specific activity.
Dependencies are inferred from the claim text.